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β‑catenin knockdown inhibits the proliferation of human glioma cells in vitro and in vivo

  • Authors:
    • Zhong Wang
    • Qianxue Chen

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  • Published online on: January 15, 2016     https://doi.org/10.3892/etm.2016.2998
  • Pages: 1059-1064
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Abstract

β‑catenin is a crucial oncogene that is capable of regulating cancer progression. The aim of the present study was to clarify whether β‑catenin was associated with the proliferation and progress of glioma. In order to knockdown the expression of β‑catenin in human U251 glioma cells, three pairs of small interfering (si)RNA were designed and synthesized and the most effective siRNA was selected and used for silencing the endogenous β‑catenin, which was detected by western blot analysis and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Proliferation was subsequently detected using a methylthiazolyl‑tetrazolium bromide assay and the results demonstrated that knockdown of β‑catenin significantly inhibited the proliferation of U251 cells in a time‑ and dose‑dependent manner (P<0.01). Cell apoptosis rate was analyzed using flow cytometry and Annexin V‑fluorescein isothiocyanate/propidium iodide staining demonstrated that β‑catenin siRNA significantly increased the apoptosis of U251 cells (P<0.01). Furthermore, the results of an in vitro scratch assay demonstrated that β‑catenin silencing suppressed the proliferation of U251 cells, as compared with the control group (P<0.01). In vivo, β‑catenin expression levels in U251 cells were significantly inhibited (P<0.01) following β‑catenin short hairpin (sh)RNA lentiviral‑vector transfection, as detected by western blot analysis and RT‑qPCR. Tumorigenicity experiments demonstrated that β‑catenin inhibition significantly increased the survival rate of nude mice. The results of the present study demonstrated that knockdown of β‑catenin expression significantly inhibited the progression of human glioma cancer cells, in vitro and in vivo; thus suggesting that β‑catenin silencing may be a novel therapy for the treatment of human glioma.
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March-2016
Volume 11 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang Z and Chen Q: β‑catenin knockdown inhibits the proliferation of human glioma cells in vitro and in vivo. Exp Ther Med 11: 1059-1064, 2016.
APA
Wang, Z., & Chen, Q. (2016). β‑catenin knockdown inhibits the proliferation of human glioma cells in vitro and in vivo. Experimental and Therapeutic Medicine, 11, 1059-1064. https://doi.org/10.3892/etm.2016.2998
MLA
Wang, Z., Chen, Q."β‑catenin knockdown inhibits the proliferation of human glioma cells in vitro and in vivo". Experimental and Therapeutic Medicine 11.3 (2016): 1059-1064.
Chicago
Wang, Z., Chen, Q."β‑catenin knockdown inhibits the proliferation of human glioma cells in vitro and in vivo". Experimental and Therapeutic Medicine 11, no. 3 (2016): 1059-1064. https://doi.org/10.3892/etm.2016.2998