Treatment of radiation‑induced acute intestinal injury with bone marrow‑derived mesenchymal stem cells

Zheng,Kai; Wu,Weizhen; Yang,Shunliang; Huang,Lianghu; Chen,Jin; Gong,Chungui; Fu,Zhichao; Lin,Ruofei; Tan,Jianming

doi:10.3892/etm.2016.3248

Treatment of radiation‑induced acute intestinal injury with bone marrow‑derived mesenchymal stem cells

Authors:
- Kai Zheng
- Weizhen Wu
- Shunliang Yang
- Lianghu Huang
- Jin Chen
- Chungui Gong
- Zhichao Fu
- Ruofei Lin
- Jianming Tan
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Affiliations: Cell and Organ Transplant Institute, Fuzhou General Hospital, Fuzhou, Fujian 350025, P.R. China, Radiotherapy Centre, Fuzhou General Hospital, Fuzhou, Fujian 350025, P.R. China
Published online on: April 11, 2016 https://doi.org/10.3892/etm.2016.3248
Pages: 2425-2431

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Abstract

The aim of the present study was to investigate the ability of bone marrow‑derived mesenchymal stem cells (BMSCs) to repair radiation‑induced acute intestinal injury, and to elucidate the underlying repair mechanism. Male Sprague‑Dawley rats were subjected to whole abdominal irradiation using a single medical linear accelerator (12 Gy) and randomly assigned to two groups. Rats in the BMSC‑treated group were injected with 1 ml BMSC suspension (2x106 cells/ml) via the tail vein, while the control group rats were injected with normal saline. BMSCs were identified by detecting the expression of CD29, CD90, CD34 and CD45 using flow cytometry. The expression of the cytokines stromal cell‑derived factor 1 (SDF‑1), prostaglandin E2 (PGE2) and interleukin (IL)‑2 was detected using immunohistochemical techniques. Plasma citrulline concentrations were evaluated using an ELISA kit. Rat general conditions, including body weight, and changes in cellular morphology were also recorded. The results suggested that BMSCs exerted a protective effect on radiation‑induced acute intestinal injury in rats. The histological damage was rapidly repaired in the BMSC‑treated group. In addition, the BMSC‑treated group showed significantly reduced radiation injury scores (P<0.01), mildly reduced body weight and plasma citrulline levels, significantly more rapid recovery (P<0.01), significantly reduced expression of the cytokines PGE2 and IL‑2 (P<0.05) and significantly increased SDF‑1 expression (P<0.01) compared with the control group. In summary, the present results indicate that BMSCs are able to effectively reduce inflammation and promote repair of the structure and function of intestinal tissues damaged by radiation exposure, suggesting that they may provide a promising therapeutic agent.

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