Open Access

Potential implications of interleukin-7 in chronic wound healing

  • Authors:
    • Annie Bartlett
    • Andrew J. Sanders
    • Fiona Ruge
    • Keith G. Harding
    • Wen G. Jiang
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  • Published online on: April 18, 2016     https://doi.org/10.3892/etm.2016.3263
  • Pages: 33-40
  • Copyright: © Bartlett et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Methods of identifying chronic wounds that will heal in a timely, coordinated fashion and those that will not, together with novel therapeutic strategies, are vital for progression in the field of wound healing. Interleukin (IL)‑7 has been associated with various biological and pathological processes. The present study explored the potential role of IL‑7 in wound healing. IL-7 expression levels were examined in a clinical cohort of chronic wounds using reverse transcription-quantitative polymerase chain reaction and immunohistochemical staining analysis. The impact of recombinant human IL‑7 (rhIL-7) on the growth and migrational rates of HaCaT keratinocyte cells was subsequently examined using in vitro growth and electric cell‑substrate impedance sensing functional assays. The mRNA expression levels of IL‑7 were increased in the healed chronic wound tissue samples, compared with non‑healed chronic wound tissue samples, although the difference was not statistically significant. Similarly, immunohistochemical analysis revealed a greater staining intensity of IL‑7 in the healed chronic wound tissue sections compared with the non‑healed tissue sections. Treatment with rhIL‑7 did not affect HaCaT cell growth rates, but was shown to enhance cell migration, an effect that could be further enhanced through the addition of inhibitors of neuronal Wiskott‑Aldrich syndrome protein and protein kinase B. The data of the present study suggest that the expression levels of IL‑7 may be increased in healing chronic wounds, and thus IL‑7 may have a role in this process, potentially through its effects on the cellular migration of keratinocytes.
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July-2016
Volume 12 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Bartlett A, Sanders AJ, Ruge F, Harding KG and Jiang WG: Potential implications of interleukin-7 in chronic wound healing. Exp Ther Med 12: 33-40, 2016.
APA
Bartlett, A., Sanders, A.J., Ruge, F., Harding, K.G., & Jiang, W.G. (2016). Potential implications of interleukin-7 in chronic wound healing. Experimental and Therapeutic Medicine, 12, 33-40. https://doi.org/10.3892/etm.2016.3263
MLA
Bartlett, A., Sanders, A. J., Ruge, F., Harding, K. G., Jiang, W. G."Potential implications of interleukin-7 in chronic wound healing". Experimental and Therapeutic Medicine 12.1 (2016): 33-40.
Chicago
Bartlett, A., Sanders, A. J., Ruge, F., Harding, K. G., Jiang, W. G."Potential implications of interleukin-7 in chronic wound healing". Experimental and Therapeutic Medicine 12, no. 1 (2016): 33-40. https://doi.org/10.3892/etm.2016.3263