Open Access

Epitope spreading induced by immunization with synthetic SSB peptides

  • Authors:
    • Min Ding
    • Jianzhong Zhang
  • View Affiliations

  • Published online on: April 19, 2016     https://doi.org/10.3892/etm.2016.3267
  • Pages: 147-150
  • Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Sjogren's syndrome type B (SSB)/La antibody is an autoantibody generally observed in connective tissue diseases whereas double-stranded deoxyribonucleic acid (dsDNA) antibodies are the most characteristic autoantibodies found in systemic lupus erythematosus (SLE) patients. The relationship of these autoantibodies remains unclear. The aim of the study was to determine the profile of antibody production in rabbits immunized with synthetic SSB peptides alone or with dsDNA. For this purpose, 214‑225aa peptide of SSB antigen was synthesized based on the organic chemistry solid‑phase peptide synthesis. Rabbits were immunized with the following antigens: i) synthetic SSB peptides linked with keyhole limpet hemocyanin (KLH); ii) dsDNA; iii) SSB plus dsDNA; iv) KLH; and v) phosphate‑buffered saline. SSB peptide antibody was measured using the enzyme‑linked immunosorbent assay while extractable nuclear antigens (ENA) antibody and dsDNA antibody were measured by immunoblotting and immunofluorescence, respectively. The results showed that a specific anti‑SSB peptide antibody was produced following immunization with SSB epitope alone or with dsDNA. The SSB peptide antibody titer in the coimmunization group was higher than that of the SSB alone group. In addition, antibodies against ribonucleoprotein (RNP), Smith and/or dsDNA were detected in rabbits of the coimmunization group. The presence of anti‑dsDNA antibodies in the rabbits immunized with SSB peptide suggested the induction of epitope spreading. In conclusions, SSB antibodies were produced in rabbits immunized with SSB peptide or SSB+dsDNA, whereas SSB antibody titers were higher in the coimmunization group. Furthermore, coimmunization was associated with epitope spreading.
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July-2016
Volume 12 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ding M and Zhang J: Epitope spreading induced by immunization with synthetic SSB peptides. Exp Ther Med 12: 147-150, 2016.
APA
Ding, M., & Zhang, J. (2016). Epitope spreading induced by immunization with synthetic SSB peptides. Experimental and Therapeutic Medicine, 12, 147-150. https://doi.org/10.3892/etm.2016.3267
MLA
Ding, M., Zhang, J."Epitope spreading induced by immunization with synthetic SSB peptides". Experimental and Therapeutic Medicine 12.1 (2016): 147-150.
Chicago
Ding, M., Zhang, J."Epitope spreading induced by immunization with synthetic SSB peptides". Experimental and Therapeutic Medicine 12, no. 1 (2016): 147-150. https://doi.org/10.3892/etm.2016.3267