Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid

Lee,Ji  Young; Park,Wansu

doi:10.3892/etm.2016.3320

Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid

Authors:
- Ji Young Lee
- Wansu Park
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Affiliations: Department of Pathology, College of Korean Medicine, Gachon University, Seongnam, Gyeonggi 461‑701, Republic of Korea
Published online on: May 9, 2016 https://doi.org/10.3892/etm.2016.3320
Pages: 151-156
Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oroxylin A (5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one; Baicalein 6-methyl ether) is an active flavonoid compound originally isolated from Scutellaria radix, which has been used to treat pulmonary infection in Korea, China, and Japan. Oroxylin A is known to possess dopamine reuptake inhibitor activity. However, the effects of oroxylin A on virus‑induced macrophages has not been fully elucidated. In the present study, the anti‑inflammatory effects of oroxylin A on double‑stranded RNA‑induced macrophages were examined. Production of nitric oxide (NO), various cytokines, as well as calcium release and the mRNA expression of signal transducer and activator of transcription 1 (STAT1) in dsRNA polyinosinic-polycytidylic acid (PIC)‑induced RAW 264.7 mouse macrophages were evaluated. Oroxylin A restored the cell viability in PIC‑induced RAW 264.7 mouse macrophages at concentrations of up to 50 µM. Additionally, oroxylin A significantly inhibited the production of nitric oxide (NO), interleukin (IL)‑1α, IL‑1β, IL‑6, IL‑10, interferon gamma‑induced protein 10, granulocyte‑colony stimulating factor (CSF), granulocyte macrophage‑CSF, leukemia inhibitory factor (IL‑6 class cytokine), lipopolysaccharide‑induced CXC chemokine (LIX), monocyte chemoattractant protein 1, macrophage inflammatory protein (MIP)‑1α, MIP‑1β, MIP‑2, Regulated on Activation, Normal T Expressed and Secreted, tumor necrosis factor‑α, and vascular endothelial growth factor as well as calcium release and the mRNA expression of STAT1 in PIC‑induced RAW 264.7 cells (P<0.05). Thus, the present results suggest that oroxylin A has anti‑inflammatory properties, associated with its inhibition of NO, cytokines, chemokines and growth factors in PIC-induced macrophages via the calcium-STAT pathway.

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