Open Access

Benexate hydrochloride betadex modulates nitric oxide synthesis and cytokine expression in gastric ulcers

  • Authors:
    • Jae Min Lee
    • Ji‑Youn Lim
    • Yoonjin Kim
    • Ye Ji Kim
    • Hyuk Soon Choi
    • Eun Sun Kim
    • Bora Keum
    • Yeon Seok Seo
    • Yoon Tae Jeen
    • Hong Sik Lee
    • Soon Ho Um
    • Chang Duck Kim
    • Ho Sang Ryu
    • Donggeun Sul
    • Junghwa Hong
    • Hoon Jai Chun
  • View Affiliations

  • Published online on: May 24, 2016     https://doi.org/10.3892/etm.2016.3384
  • Pages: 573-580
  • Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated benexate hydrochloride betadex (BHB)-mediated ulcer healing, and changes to microcirculation modulated through nitric oxide synthase (NOS) and anti‑inflammatory activity. A rat model of gastric mucosal injury was established through injection of a 60% acetic acid solution into the stomach. Following ulcer induction, the rats were administered BHB orally for 5 days at doses of 0, 100, 300 or 1,000 mg/kg. The highest dose of BHB was also administered with or without L-NG-nitroarginine methyl ester (L‑NAME). The area of gastric ulcers was determined by planimetry, and expression of cyclooxygenases (COX), cytokines and NOS in stomach tissues were measured using western blotting. Compared with the control group, gastric ulcer size was significantly decreased in the 1,000 mg/kg BHB‑treated group (P<0.05). Administration of BHB led to a significant increase in endothelial (e)NOS expression (P<0.05). Although acetic acid co‑treatment with L‑NAME induced more severe mucosal damage, BHB decreased COX expression and tumor necrosis factor‑α levels when administered with the nitric oxide inhibitor, L-NAME (P<0.05). BHB exhibited protective effects in a rat model of gastric ulcers, which were associated with a decrease in pro‑inflammatory cytokine levels and the activation of eNOS.
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August-2016
Volume 12 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Lee JM, Lim JY, Kim Y, Kim YJ, Choi HS, Kim ES, Keum B, Seo YS, Jeen YT, Lee HS, Lee HS, et al: Benexate hydrochloride betadex modulates nitric oxide synthesis and cytokine expression in gastric ulcers. Exp Ther Med 12: 573-580, 2016.
APA
Lee, J.M., Lim, J., Kim, Y., Kim, Y.J., Choi, H.S., Kim, E.S. ... Chun, H.J. (2016). Benexate hydrochloride betadex modulates nitric oxide synthesis and cytokine expression in gastric ulcers. Experimental and Therapeutic Medicine, 12, 573-580. https://doi.org/10.3892/etm.2016.3384
MLA
Lee, J. M., Lim, J., Kim, Y., Kim, Y. J., Choi, H. S., Kim, E. S., Keum, B., Seo, Y. S., Jeen, Y. T., Lee, H. S., Um, S. H., Kim, C. D., Ryu, H. S., Sul, D., Hong, J., Chun, H. J."Benexate hydrochloride betadex modulates nitric oxide synthesis and cytokine expression in gastric ulcers". Experimental and Therapeutic Medicine 12.2 (2016): 573-580.
Chicago
Lee, J. M., Lim, J., Kim, Y., Kim, Y. J., Choi, H. S., Kim, E. S., Keum, B., Seo, Y. S., Jeen, Y. T., Lee, H. S., Um, S. H., Kim, C. D., Ryu, H. S., Sul, D., Hong, J., Chun, H. J."Benexate hydrochloride betadex modulates nitric oxide synthesis and cytokine expression in gastric ulcers". Experimental and Therapeutic Medicine 12, no. 2 (2016): 573-580. https://doi.org/10.3892/etm.2016.3384