Effects of testosterone on the expression levels of AMH, VEGF and HIF‑1α in mouse granulosa cells

Zhang,Ying; Wang,Shan‑Feng; Zheng,Jin‑Dan; Zhao,Chun‑Bo; Zhang,Yi‑Nan; Liu,Li‑Li; Huang,Jian‑Hua

doi:10.3892/etm.2016.3436

Effects of testosterone on the expression levels of AMH, VEGF and HIF‑1α in mouse granulosa cells

Authors:
- Ying Zhang
- Shan‑Feng Wang
- Jin‑Dan Zheng
- Chun‑Bo Zhao
- Yi‑Nan Zhang
- Li‑Li Liu
- Jian‑Hua Huang
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Affiliations: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Key Laboratory of Tissue Engineering, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China
Published online on: June 8, 2016 https://doi.org/10.3892/etm.2016.3436
Pages: 883-888
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the effects of testosterone on mouse granulosa cell morphology, and the expression levels of anti‑Müllerian hormone (AMH), vascular endothelial growth factor (VEGF) and hypoxia‑inducible factor‑1α (HIF‑1α). Mouse granulosa cells were isolated and identified, and their morphology was examined using hematoxylin and eosin, F‑actin, and follicle‑stimulating hormone receptor staining. The mRNA expression levels of AMH, VEGF and HIF‑1α were examined using reverse transcription‑quantitative polymerase chain reaction, and their protein secretion levels were investigated using enzyme‑linked immunosorbent assays. Testosterone treatment did not affect granulosa cell morphology; however, it significantly increased the mRNA expression levels of AMH and VEGF, and the protein secretion levels of AMH, VEGF and HIF‑1α. These results suggested that testosterone was able to regulate the functions of granulosa cells by upregulating the expression levels of AMH, VEGF and HIF‑1α.

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