Effects of the κ‑opioid receptor on the inhibition of 100 Hz electroacupuncture on cocaine‑induced conditioned place preference

Hou,Bingjun

doi:10.3892/etm.2016.3504

Effects of the κ‑opioid receptor on the inhibition of 100 Hz electroacupuncture on cocaine‑induced conditioned place preference

Authors:
- Bingjun Hou
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Affiliations: Basic Department, Shandong Medical College, Linyi, Shandong 276000, P.R. China
Published online on: July 5, 2016 https://doi.org/10.3892/etm.2016.3504
Pages: 1604-1610

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Abstract

The administration of 100 Hz electroacupuncture has been demonstrated to suppress cocaine‑induced conditioned place preference (CPP) in rats, and there is evidence that the κ‑opioid receptor may have a role in cocaine addiction. The present study sought to explore the mechanisms underlying the inhibitory effects of 100 Hz electroacupuncture on cocaine‑induced CPP in rats. A rat model of cocaine‑induced CPP was used in the present study to investigate the following: i) Naloxone treatment (5 and 10 mg/kg) following 100 Hz electroacupuncture‑mediated inhibition on cocaine‑induced CPP, revealing that a high dose (10 mg/kg) of naloxone blocked the inhibitory effects of 100 Hz electroacupuncture on cocaine‑induced CPP; ii) nor‑binaltorphimine (nor‑BNI) on 100 Hz electroacupuncture‑mediated inhibition on cocaine‑induced CPP, which indicated that administration of 10 µg/5 µl and 0.3 µg/1 µl nor‑BNI intracerebroventricularly and via the nucleus accumbens, respectively, reversed the inhibitory effects of 100 Hz electroacupuncture on cocaine‑induced CPP, and that injection of nor‑BNI in different brain areas of rats blocks the inhibitory effects of electroacupuncture on cocaine‑induced CPP; and iv) 100 Hz electroacupuncture on the mRNA expression levels of the κ‑opioid receptor in the rat nucleus accumbens and amygdala, which established that mRNA expression levels of κ‑opioid receptor in the nucleus accumbens were increased with 100 Hz electroacupuncture plus cocaine‑induced CPP. Overall, the results of the present study indicated that 100 Hz electroacupuncture was able to suppress cocaine‑induced CPP via the κ‑opioid receptor in the nucleus accumbens.

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