H4K5 histone acetylation of BRG1 is associated with heroin administration rather than addiction

  • Authors:
    • Limin Xu
    • Qingxiao Hong
    • Xiaoying Chen
    • Xuting Xu
    • Huifen Liu
    • Wenhua Zhou
    • Shiwei Duan
  • View Affiliations

  • Published online on: July 13, 2016     https://doi.org/10.3892/etm.2016.3517
  • Pages: 1929-1933
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Abstract

Diacetylmorphine hydrochloride (heroin) addiction is a chronic relapsing brain disorder that is a heavy public health burden worldwide. Brm/SWI2-related gene-1 (BRG1) is a tumor suppressor gene that can influence embryogenesis and the development of the cerebellum. The current study aimed to investigate the effect of histone H4 lysine 5 (H4K5) modifications on the BRG1 gene in brain tissue of the ventral tegmental area (VTA) of heroin‑addicted rats. A total of 21 male Sprague Dawley rats were raised in a standard manner and underwent heroin self‑administration training. Rats were randomly divided into three equal groups: Group A, self-administered delivery of heroin; group B, yoked delivery of heroin; and group C, yoked delivery of saline. The VTA was harvested and subjected to chromatin immunoprecipitation (ChIP) analysis. Gene expression was evaluated by quantitative polymerase chain reaction. We calculated the recovery rate, which indicated the percentage of the total input BRG1 recovered by ChIP. Our results showed that BRG1 was less associated with H4K5 histone modification in the group of rats that underwent heroin self‑administration than in the other two groups (A vs. B, P=0.031; A vs. C, P=0.067). The recovery fold changes of the self‑administration group and the passive-administration group were significantly different from those of the group with yoked saline (A vs. C, P=0.013; B vs. C, P=0.009; A vs. B, P=0.731). The results of the current study demonstrated that H4K5 histone acetylation of BRG1 in the VTA may be associated with heroin administration, but not addiction.
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September-2016
Volume 12 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Xu L, Hong Q, Chen X, Xu X, Liu H, Zhou W and Duan S: H4K5 histone acetylation of BRG1 is associated with heroin administration rather than addiction. Exp Ther Med 12: 1929-1933, 2016.
APA
Xu, L., Hong, Q., Chen, X., Xu, X., Liu, H., Zhou, W., & Duan, S. (2016). H4K5 histone acetylation of BRG1 is associated with heroin administration rather than addiction. Experimental and Therapeutic Medicine, 12, 1929-1933. https://doi.org/10.3892/etm.2016.3517
MLA
Xu, L., Hong, Q., Chen, X., Xu, X., Liu, H., Zhou, W., Duan, S."H4K5 histone acetylation of BRG1 is associated with heroin administration rather than addiction". Experimental and Therapeutic Medicine 12.3 (2016): 1929-1933.
Chicago
Xu, L., Hong, Q., Chen, X., Xu, X., Liu, H., Zhou, W., Duan, S."H4K5 histone acetylation of BRG1 is associated with heroin administration rather than addiction". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1929-1933. https://doi.org/10.3892/etm.2016.3517