Separation of cordycepin from Cordyceps militaris fermentation supernatant using preparative HPLC and evaluation of its antibacterial activity as an NAD+-dependent DNA ligase inhibitor

Zhou,Xiaofeng; Cai,Guoqiang; He,Yi; Tong,Guotong

doi:10.3892/etm.2016.3536

Separation of cordycepin from Cordyceps militaris fermentation supernatant using preparative HPLC and evaluation of its antibacterial activity as an NAD+-dependent DNA ligase inhibitor

Authors:
- Xiaofeng Zhou
- Guoqiang Cai
- Yi He
- Guotong Tong
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Affiliations: Key Laboratory of Green Chemical Engineering, Linjing College, Hangzhou Vocational and Technical College, Hangzhou, Zhejiang 310018, P.R. China, Department of Traditional Chinese Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310018, P.R. China
Published online on: July 20, 2016 https://doi.org/10.3892/etm.2016.3536
Pages: 1812-1816

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Abstract

Cordycepin exhibits various bio-activities, including anticancer, antibacterial, antiviral and immune regulation activities, and is a significant focus of research. However, the preparation of high-purity cordycepin remains challenging. Also, the molecular target with which cordycepin interacts to cause an antibacterial effect remains unknown. In the present study, cordycepin was prepared by preparative high‑performance liquid chromatography (prep-HPLC) and the purity obtained was 99.6%, indicating that this technique may be useful for the large‑scale isolation of cordycepin in the future. The results of computational molecular docking analysis indicated that the interaction energy between cordycepin and NAD+‑dependent DNA ligase (LigA) was lower than that between cordycepin and other common antibacterial targets. The highly pure cordycepin obtained by prep‑HPLC demonstrated inhibitory activity against LigA from various bacteria in vitro. In conclusion, cordycepin may be useful as a broad‑spectrum antibiotic targeting LigA in various bacteria.

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