Increased receptor activator of nuclear factor κβ ligand/osteoprotegerin ratio exacerbates cartilage destruction in osteoarthritis in vitro

  • Authors:
    • Ji‑Zhou Zeng
    • Zhen‑Zhong Wang
    • Li‑Feng Ma
    • Hai Meng
    • Hao‑Miao Yu
    • Wen‑Hao Cheng
    • Ya‑Kui Zhang
    • Ai Guo
  • View Affiliations

  • Published online on: August 31, 2016     https://doi.org/10.3892/etm.2016.3638
  • Pages: 2778-2782
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Abstract

Osteoarthritis (OA) is a degenerative joint disease characterized by progressive cartilage destruction, matrix degradation and bony changes. Subchondral bone alterations in osteoarthritis are associated with cartilage destruction. It has previously been demonstrated that osteoprotegerin (OPG) and receptor activator of nuclear factor κβ ligand (RANKL) mediate this process. The RANKL/OPG ratio is altered in OA chondrocytes compared with normal chondrocytes. In the pathogenesis of OA, abnormal expression levels of matrix metalloproteinase‑13 (MMP‑13) are secreted by chondrocytes has a vital role in the progression of cartilage erosion. In the present study, the effect of various RANKL/OPG ratios on MMP‑13 expression levels was investigated in interleukin‑1β‑stimulated SW1353 human chondrosarcoma cells. Cell viability was assessed by MTT assay and MMP‑13 mRNA and protein expression levels were analyzed by quantitative reverse-transcription-quantitative polymerase chain reaction, ELISA and western blot analyses, respectively. The results demonstrated that an increase in MMP‑13 mRNA and protein expression levels was observed with increasing RANKL/OPG ratio. These findings suggest that this mechanism may be used as a novel therapeutic strategy against OA.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zeng JZ, Wang ZZ, Ma LF, Meng H, Yu HM, Cheng WH, Zhang YK and Guo A: Increased receptor activator of nuclear factor κβ ligand/osteoprotegerin ratio exacerbates cartilage destruction in osteoarthritis in vitro. Exp Ther Med 12: 2778-2782, 2016.
APA
Zeng, J., Wang, Z., Ma, L., Meng, H., Yu, H., Cheng, W. ... Guo, A. (2016). Increased receptor activator of nuclear factor κβ ligand/osteoprotegerin ratio exacerbates cartilage destruction in osteoarthritis in vitro. Experimental and Therapeutic Medicine, 12, 2778-2782. https://doi.org/10.3892/etm.2016.3638
MLA
Zeng, J., Wang, Z., Ma, L., Meng, H., Yu, H., Cheng, W., Zhang, Y., Guo, A."Increased receptor activator of nuclear factor κβ ligand/osteoprotegerin ratio exacerbates cartilage destruction in osteoarthritis in vitro". Experimental and Therapeutic Medicine 12.4 (2016): 2778-2782.
Chicago
Zeng, J., Wang, Z., Ma, L., Meng, H., Yu, H., Cheng, W., Zhang, Y., Guo, A."Increased receptor activator of nuclear factor κβ ligand/osteoprotegerin ratio exacerbates cartilage destruction in osteoarthritis in vitro". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2778-2782. https://doi.org/10.3892/etm.2016.3638