Identification of the protective effects of traditional medicinal plants against SDS-induced Drosophila gut damage

Zhou,Yang; Liu,Zonglin; Chen,Yuchen; Jin,Li  Hua

doi:10.3892/etm.2016.3641

Identification of the protective effects of traditional medicinal plants against SDS-induced Drosophila gut damage

Authors:
- Yang Zhou
- Zonglin Liu
- Yuchen Chen
- Li Hua Jin
View Affiliations

Affiliations: College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang 150040, P.R. China
Published online on: August 31, 2016 https://doi.org/10.3892/etm.2016.3641
Pages: 2671-2680

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Traditional medicinal plants are widely used as immunomodulatory medicines that help improve health. A total of 50 different plants used for the treatment of toxicity were screened for their in vivo protective effects. Flies were fed a standard cornmeal-yeast medium (control group) or the standard medium containing medicinal plant extracts (experimental groups). Assessment of the survival rate was performed by feeding flies with toxic compounds. Gut epithelial cells were analyzed for cell proliferation and death by green fluorescent protein antibodies and 7‑aminoactinomycin D staining under the microscope. The expression of antimicrobial peptides (AMPs) was evaluated by the quantitative polymerase chain reaction and the results revealed that after feeding the flies with toxic compounds, aqueous extracts from Codonopsis pilosula (Franch.) Nannf (C. pilosula), Saussurea lappa (Decne.) C.B.Clarke (S. lappa), Imperata cylindrica Beauv.var.major (Nees) C.E. Hubb. (I. cylindrical var. major) and Melia toosendan Sied. Et Zucc. (M.toosendan) increased the fly survival rate, reduced epithelial cell death and improved gut morphology. In addition, C. pilosula extracts induced the antimicrobial peptide levels (Dpt and Mtk) following treatment with sodium dodecyl sulfate (SDS). However, these extracts were not observed to increase SDS‑induced cell proliferation in vivo. These results indicate that there are strong protective effects in extracts of C. pilosula, S. lappa, I. cylindrical var. major and M. toosendan on Drosophila intestinal cells among 50 medicinal plants.

View Figures

View References

1	Gautam R and Jachak SM: Recent developments in anti-inflammatory natural products. Med Res Rev. 29:767–820. 2009. View Article : Google Scholar : PubMed/NCBI
2	Lee KA and Lee WJ: Drosophila as a model for intestinal dysbiosis and chronic inflammatory diseases. Dev Comp Immunol. 42:102–110. 2014. View Article : Google Scholar : PubMed/NCBI
3	Chatterjee M and Ip YT: Pathogenic stimulation of intestinal stem cell response in Drosophila. J Cell Physiol. 220:664–671. 2009. View Article : Google Scholar : PubMed/NCBI
4	Micchelli CA and Perrimon N: Evidence that stem cells reside in the adult Drosophila midgut epithelium. Nature. 439:475–479. 2006. View Article : Google Scholar : PubMed/NCBI
5	Jiang H, Patel PH, Kohlmaier A, Grenley MO, McEwen DG and Edgar BA: Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut. Cell. 137:1343–1355. 2009. View Article : Google Scholar : PubMed/NCBI
6	Ha EM, Oh CT, Bae YS and Lee WJ: A direct role for dual oxidase in Drosophila gut immunity. Science. 310:847–850. 2005. View Article : Google Scholar : PubMed/NCBI
7	Ryu JH, Ha EM, Oh CT, Seol JH, Brey PT, Jin I, Lee DG, Kim J, Lee D and Lee WJ: An essential complementary role of NF-kappaB pathway to microbicidal oxidants in Drosophila gut immunity. EMBO J. 25:3693–3701. 2006. View Article : Google Scholar : PubMed/NCBI
8	Lenaz G: Role of mitochondria in oxidative stress and ageing. Biochim Biophys Acta. 1366:53–67. 1998. View Article : Google Scholar : PubMed/NCBI
9	Liehl P, Blight M, Vodovar N, Boccard F and Lemaitre B: Prevalence of local immune response against oral infection in a Drosophila/Pseudomonas infection model. PLoS Pathog. 2:e562006. View Article : Google Scholar : PubMed/NCBI
10	Hu Y, Wang S, Wu X, Zhang J, Chen R, Chen M and Wang Y: Chinese herbal medicine-derived compounds for cancer therapy: A focus on hepatocellular carcinoma. J Ethnopharmacol. 149:601–612. 2013. View Article : Google Scholar : PubMed/NCBI
11	Li W, Luo Q and Jin LH: Acanthopanax senticosus extracts have a protective effect on Drosophila gut immunity. J Ethnopharmacol. 146:257–263. 2013. View Article : Google Scholar : PubMed/NCBI
12	Steele ML, Truong J, Govindaraghavan S, Ooi L, Sucher NJ and Münch G: Cytoprotective properties of traditional chinese medicinal herbal extracts in hydrogen peroxide challenged human U373 astroglia cells. Neurochem Int. 62:522–529. 2013. View Article : Google Scholar : PubMed/NCBI
13	Seisenbacher G, Hafen E and Stocker H: MK2-dependent p38b signalling protects Drosophila hindgut enterocytes against JNK-induced apoptosis under chronic stress. PLoS Genet. 7:e10021682011. View Article : Google Scholar : PubMed/NCBI
14	Amcheslavsky A, Jiang J and Ip YT: Tissue damage-induced intestinal stem cell division in Drosophila. Cell Stem Cell. 4:49–61. 2009. View Article : Google Scholar : PubMed/NCBI
15	Kawada M, Arihiro A and Mizoguchi E: Insights from advances in research of chemically induced experimental models of human inflammatory bowel disease. World J Gastroenterol. 13:5581–5593. 2007. View Article : Google Scholar : PubMed/NCBI
16	Buchon N, Broderick NA, Poidevin M, Pradervand S and Lemaitre B: Drosophila intestinal response to bacterial infection: Activation of host defense and stem cell proliferation. Cell Host Microbe. 5:200–211. 2009. View Article : Google Scholar : PubMed/NCBI
17	Yu L, Qin Y, Wang Q, Zhang L, Liu Y, Wang T, Huang L, Wu L and Xiong H: The efficacy and safety of Chinese herbal medicine, Rhodiola formulation in treating ischemic heart disease: A systematic review and meta-analysis of randomized controlled trials. Complement Ther Med. 22:814–825. 2014. View Article : Google Scholar : PubMed/NCBI
18	Jiang Su New Medical University, . Dictionary of Chinese Traditional Medicine. 2:18371996.
19	Ng TB, Liu F and Wang HX: The antioxidant effects of aqueous and organic extracts of Panax quinquefolium, Panax notoginseng, Codonopsis pilosula, Pseudostellaria heterophylla and Glehnia littoralis. J Ethnopharmacol. 93:285–288. 2004. View Article : Google Scholar : PubMed/NCBI
20	Singh B, Song H, Liu X, Hardy M, Liu GZ, Vinjamury SP and Martironsian CD: Dangshen (Codonopsis pilosula) and Bai guo (Gingko biloba) enhance learning and memory. Altern Ther Health Med. 10:52–56. 2004.PubMed/NCBI
21	Xin T, Zhang F, Jiang Q, Chen C, Huang D, Li Y, Shen W, Jin Y and Sui G: The inhibitory effect of a polysaccharide from Codonopsis pilosula on tumor growth and metastasis in vitro. Int J Biol Macromol. 51:788–793. 2012. View Article : Google Scholar : PubMed/NCBI
22	Wang ZT, Ma JY, Tu PF and Ng TB: Chemotaxonomic study of Codonopsis (Family Campanulaceae) and its related Genera. Biochem Syst Ecol. 23:809–812. 1995. View Article : Google Scholar
23	Choi HG, Lee DS, Li B, Choi YH, Lee SH and Kim YC: Santamarin, a sesquiterpene lactone isolated from Saussurea lappa, represses LPS-induced inflammatory responses via expression of heme oxygenase-1 in murine macrophage cells. Int Immunopharmacol. 13:271–279. 2012. View Article : Google Scholar : PubMed/NCBI
24	Zhang T, Ma L, Wu F and Chen R: Chemical constituents from a portion of ethanolic extract of Saussurea lappa roots. Zhongguo Zhong Yao Za Zhi. 37:1232–1236. 2012.(In Chinese). PubMed/NCBI
25	Pharmacopeia Committee of P.R. China, . Pharmacopoeia of People's Republic of China. China Medical Science and Technology Press; Beijing: pp. 992010
26	Yoon JS, Lee MK, Sung SH and Kim YC: Neuroprotective 2-(2-phenylethyl) chromones of Imperata cylindrica. J Nat Prod. 69:290–291. 2006. View Article : Google Scholar : PubMed/NCBI
27	Xie F, Zhang M, Zhang CF, Wang ZT, Yu BY and Kou JP: Anti-inflammatory and analgesic activities of ethanolic extract and two limonoids from Melia toosendan fruit. J Ethnopharmacol. 117:463–466. 2008. View Article : Google Scholar : PubMed/NCBI
28	Lin QY, Jin LJ, Cao ZH, Lu YN, Xue HY and Xu YP: Acanthopanax senticosus suppresses reactive oxygen species production by mouse peritoneal macrophages in vitro and in vivo. Phytother Res. 22:740–745. 2009. View Article : Google Scholar
29	Zhang S, Qi Y, Xu YW, Han X, Peng JY, Liu KX and Sun CK: Protective effect of flavonoid-rich extract from Rosa laevigata Michx on cerebral ischemia-reperfusion injury through suppression of apoptosis and inflammation. Neurochem Int. 63:522–532. 2013. View Article : Google Scholar : PubMed/NCBI
30	Kim SH and Lee WJ: Role of DUOX in gut inflammation: Lessons from Drosophila model of gut-microbiota interactions. Front Cell Infect Microbiol. 3:1162014. View Article : Google Scholar : PubMed/NCBI
31	Wu Y, Antony S, Meitzler JL and Doroshow JH: Molecular mechanisms underlying chronic inflammation-associated cancers. Cancer Lett. 345:164–173. 2014. View Article : Google Scholar : PubMed/NCBI