Effect of pioglitazone on neuropathic pain and spinal expression of TLR-4 and cytokines

  • Authors:
    • Hongbin Jia
    • Shuangshuang Xu
    • Qingzhen Liu
    • Jian Liu
    • Jianguo Xu
    • Weiyan Li
    • Yi Jin
    • Qing Ji
  • View Affiliations

  • Published online on: September 1, 2016     https://doi.org/10.3892/etm.2016.3643
  • Pages: 2644-2650
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Abstract

The molecular mechanisms underlying neuropathic pain have yet to be elucidated. The present study aimed to examine the modulation of neuroimmune activation in the spinal cord by the synthetic peroxisome proliferator‑activated receptor gamma (PPAR-γ) agonist, pioglitazone (Pio), in a rat model of neuropathic pain induced by chronic constriction injury (CCI). Rats were randomly assigned into four groups: Sham surgery with vehicle, chronic constriction injury with vehicle or Pio (10 mg/kg), and chronic constriction injury with Pio and a PPAR‑γ antagonist GW9662 (2 mg/kg). Pio or vehicle was administered 1 h prior to the surgery and continued daily until day 7 post‑surgery. Paw pressure threshold was measured prior to surgery and on days 0, 1, 3 and 7 post‑surgery. Microglia activation markers macrophage antigen complex‑1, the mRNA expression levels of tumor necrosis factor α and interleukin‑1β, and the mRNA expression levels of toll like receptor (TLR‑4) in the lumbar spinal cord were determined. Administration of Pio resulted in the prominent attenuation of mechanical hyperalgesia. In addition, Pio was able to significantly inhibit neuroimmune activation characterized by glial activation, the production of cytokines and expression levels of TLR‑4. Concurrent administration of a PPAR‑γ antagonist, GW9662, reversed the effects of Pio. The antihyperalgesic effect of administration of Pio in rats receiving CCI may, in part, be attributed to the inhibition of neuroimmune activation associated with the sustaining of neuropathic pain.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Jia H, Xu S, Liu Q, Liu J, Xu J, Li W, Jin Y and Ji Q: Effect of pioglitazone on neuropathic pain and spinal expression of TLR-4 and cytokines. Exp Ther Med 12: 2644-2650, 2016.
APA
Jia, H., Xu, S., Liu, Q., Liu, J., Xu, J., Li, W. ... Ji, Q. (2016). Effect of pioglitazone on neuropathic pain and spinal expression of TLR-4 and cytokines. Experimental and Therapeutic Medicine, 12, 2644-2650. https://doi.org/10.3892/etm.2016.3643
MLA
Jia, H., Xu, S., Liu, Q., Liu, J., Xu, J., Li, W., Jin, Y., Ji, Q."Effect of pioglitazone on neuropathic pain and spinal expression of TLR-4 and cytokines". Experimental and Therapeutic Medicine 12.4 (2016): 2644-2650.
Chicago
Jia, H., Xu, S., Liu, Q., Liu, J., Xu, J., Li, W., Jin, Y., Ji, Q."Effect of pioglitazone on neuropathic pain and spinal expression of TLR-4 and cytokines". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2644-2650. https://doi.org/10.3892/etm.2016.3643