Expression and regulation of the ery operon of Brucella melitensis in human trophoblast cells

Zhang,Hui; Dou,Xiaoxia; Li,Zhiqiang; Zhang,Yu; Zhang,Jing; Guo,Fei; Wang,Yuanzhi; Wang,Zhen; Li,Tiansen; Gu,Xinli; Chen,Chuangfu

doi:10.3892/etm.2016.3688

Expression and regulation of the ery operon of Brucella melitensis in human trophoblast cells

Authors:
- Hui Zhang
- Xiaoxia Dou
- Zhiqiang Li
- Yu Zhang
- Jing Zhang
- Fei Guo
- Yuanzhi Wang
- Zhen Wang
- Tiansen Li
- Xinli Gu
- Chuangfu Chen
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Affiliations: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China, School of Life Sciences, Shangqiu Normal University, Shangqiu, Henan 476000, P.R. China, Co‑Innovation Center for Zoonotic Infectious Diseases in The Western Region, Shihezi University, Shihezi, Xinjiang 832000, P.R. China
Published online on: September 8, 2016 https://doi.org/10.3892/etm.2016.3688
Pages: 2723-2728

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Abstract

Brucellosis is primarily a disease of domestic animals in which the bacteria localizes to fetal tissues such as embryonic trophoblast cells and fluids containing erythritol, which stimulates Brucella spp. growth. The utilization of erythritol is a characteristic of the genus Brucella. The ery operon contains four genes (eryA, eryB, eryC and eryD) for the utilization of erythritol, and plays a major role in the survival and multiplication of Brucella spp. The objective of the present study was to conduct a preliminary characterization of differential genes expression of the ery operon at several time points after Brucella infected embryonic trophoblast cells (HPT‑8 cells). The result showed that the ery operon expression was higher in HPT‑8 cells compared with the medium. The relative expression of eryA, eryB and eryC peaked at 2 h post‑infection in HPT‑8 cells, and eryD expression peaked at 3 h post‑infection. The expression of eryA, eryB and eryC may be inhibited by increased eryD expression. However, the expression of the ery operon was stable in the presence of erythritol in cells. 2308Δery and 027Δery mutants of the ery operon were successfully constructed by homologous recombination, which were attenuated in RAW 264.7 murine macrophages. The characterization of the ery operon genes and their expression proﬁles in response to Brucella infection further contributes to our understanding of the molecular mechanisms of infection and the pathogenesis of brucellosis.

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