Effects of dexpanthenol on acetic acid‑induced colitis in rats

Cagin,Yasir Furkan; Parlakpinar,Hakan; Vardi,Nigar; Polat,Alaadin; Atayan,Yahya; Erdogan,Mehmet Ali; Tanbek,Kevser

doi:10.3892/etm.2016.3728

Effects of dexpanthenol on acetic acid‑induced colitis in rats

Authors:
- Yasir Furkan Cagin
- Hakan Parlakpinar
- Nigar Vardi
- Alaadin Polat
- Yahya Atayan
- Mehmet Ali Erdogan
- Kevser Tanbek
View Affiliations

Affiliations: Department of Gastroenterology, Faculty of Medicine, Inonu University, Malatya 44280, Turkey, Department of Pharmacology, Faculty of Medicine, Inonu University, Malatya 44280, Turkey, Department of Histology and Embryology, Faculty of Medicine, Inonu University, Malatya 44280, Turkey, Department of Physiology, Faculty of Medicine, Inonu University, Malatya 44280, Turkey
Published online on: September 20, 2016 https://doi.org/10.3892/etm.2016.3728
Pages: 2958-2964
Copyright: © Cagin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

While the pathogenesis of acetic acid (AA)‑induced colitis is unclear, reactive oxygen species are considered to have a significant effect. The aim of the present study was to elucidate the therapeutic potential of dexpanthenol (Dxp) on the amelioration of colitis in rats. Group I (n=8; control group) was intrarectally administered 1 ml saline solution (0.9%); group II [n=8; AA] was administered 4% AA into the colon via the rectum as a single dose for three consecutive days; group III (n=8; AA + Dxp) was administered AA at the same dosage as group II from day 4, and a single dose of Dxp was administered intraperitoneally; and group IV (n=8; Dxp) was administered Dxp similarly to Group III. Oxidative stress and colonic damage were assessed via biochemical and histologic examination methods. AA treatment led to an increase in oxidative parameters and a decrease in antioxidant systems. Histopathological examination showed that AA treatment caused tissue injury and increased caspase‑3 activity in the distal colon and triggered apoptosis. Dxp treatment caused biochemical and histopathological improvements, indicating that Dxp may have an anti‑oxidant effect in colitis; therefore, Dxp may be a potential therapeutic agent for the amelioration of IBD.

View Figures

View References

1	Khor B, Gardet A and Xavier RJ: Genetics and pathogenesis of inflammatory bowel disease. Nature. 474:307–317. 2011. View Article : Google Scholar : PubMed/NCBI
2	Karlinger K, Györke T, Makö E, Mester A and Tarján Z: The epidemiology and the pathogenesis of inflammatory bowel disease. Eur J Radiol. 35:154–167. 2000. View Article : Google Scholar : PubMed/NCBI
3	Pavlick KP, Laroux FS, Fuseler J, Wolf RE, Gray L, Hoffman J and Grisham MB: Role of reactive metabolites of oxygen and nitrogen in inflammatory bowel disease. Free Radic Biol Med. 33:311–322. 2002. View Article : Google Scholar : PubMed/NCBI
4	Harputluoglu M, Demirel U, Yücel N, Karadağ N, Temel I, Firat S, Ara C, Aladağ M, Karincaoğlu M and Hilmioğlu F: The effects of Gingko biloba extract on acetic acid-induced colitis in rats. Turk J Gastroenterol. 17:177–182. 2006.PubMed/NCBI
5	Zhu H and Li YR: Oxidative stress and redox signaling mechanisms of inflammatory bowel disease: Updated experimental and clinical evidence. Exp Biol Med (Maywood). 237:474–480. 2012. View Article : Google Scholar : PubMed/NCBI
6	Pereira C, Grácio D, Teixeira JP and Magro F: Oxidative stress and DNA damage: Implications in inflammatory bowel disease. Inflamm Bowel Dis. 21:2403–2417. 2015.PubMed/NCBI
7	Vishwakarma N, Ganeshpurkar A, Pandey V, Dubey N and Bansal D: Mesalazine-probiotics beads for acetic acid experimental colitis: Formulation and characterization of a promising new therapeutic strategy for ulcerative colitis. Drug Deliv. 22:94–99. 2015. View Article : Google Scholar : PubMed/NCBI
8	Slyshenkov VS, Rakowska M, Moiseenok AG and Wojtczak L: Pantothenic acid and its derivatives protect Ehrlich ascites tumor cells against lipid peroxidation. Free Radic Biol Med. 19:767–772. 1995. View Article : Google Scholar : PubMed/NCBI
9	Wojtczak L and Slyshenkov VS: Protection by pantothenic acid against apoptosis and cell damage by oxygen free radicals-the role of glutathione. Biofactors. 17:61–73. 2003. View Article : Google Scholar : PubMed/NCBI
10	Jessop CE and Bulleid NJ: Glutathione directly reduces an oxidoreductase in the endoplasmic reticulum of mammalian cells. J Biol Chem. 279:55341–55347. 2004. View Article : Google Scholar : PubMed/NCBI
11	Ceylan H, Yapici S, Tutar E, Ceylan NO, Tarakçıoğlu M and Demiryurek AT: Protective effects of dexpanthenol and y-27632 on stricture formation in a rat model of caustic esophageal injury. J Surg Res. 171:517–523. 2011. View Article : Google Scholar : PubMed/NCBI
12	Arribas B, Suárez-Pereira E, Mellet C Ortiz, García Fernández JM, Buttersack C, Rodríguez-Cabezas ME, Garrido-Mesa N, Bailon E, Guerra-Hernández E, Zarzuelo A and Gálvez J: Di-D-fructose dianhydride-enriched caramels: Effect on colon microbiota, inflammation, and tissue damage in trinitrobenzenesulfonic acid-induced colitic rats. J Agric Food Chem. 58:6476–6484. 2010. View Article : Google Scholar : PubMed/NCBI
13	Mihara M and Uchiyama M: Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 86:271–278. 1978. View Article : Google Scholar : PubMed/NCBI
14	Lowry OH, Rosebrough NJ, Farr AL and Randall RJ: Protein measurement with the Folin phenol reagent. J biol Chem. 193:265–275. 1951.PubMed/NCBI
15	Sun Y, Oberley LW and Li Y: A simple method for clinical assay of superoxide dismutase. Clin Chem. 34:497–500. 1988.PubMed/NCBI
16	Aebi H and Suter H: CatalaseMethods of Enzymatic Analysis. Bergmeyer HU: Academic Press; New York, NY: pp. 3251969
17	Paglia DE and Valentine WN: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 70:158–169. 1967.PubMed/NCBI
18	Erel O: A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 37:112–119. 2004. View Article : Google Scholar : PubMed/NCBI
19	Burisch J, Jess T, Martinato M and Lakatos PL: ECCO-EpiCom: The burden of inflammatory bowel disease in Europe. J Crohns Colitis. 7:322–337. 2013. View Article : Google Scholar : PubMed/NCBI
20	Martinez CA, Ribeiro ML, Gambero A, Miranda DD, Pereira JA and Nadal SR: The importance of oxygen free radicals in the etiopathogenesis of diversion colitis in rats. Acta Cir Bras. 25:387–395. 2010. View Article : Google Scholar : PubMed/NCBI
21	Kim YJ, Kim EH and Hahm KB: Oxidative stress in inflammation-based gastrointestinal tract diseases: Challenges and opportunities. J Gastroenterol Hepatol. 27:1004–1010. 2012. View Article : Google Scholar : PubMed/NCBI
22	Thompson CB: Apoptosis in the pathogenesis and treatment of disease. Science. 267:1456–1462. 1995. View Article : Google Scholar : PubMed/NCBI
23	Franco R, Sánchez-Olea R, Reyes-Reyes EM and Panayiotidis MI: Environmental toxicity, oxidative stress and apoptosis: Ménage à trois. Mutat Res. 674:3–22. 2009. View Article : Google Scholar : PubMed/NCBI
24	Souza HS, Tortori CJ, Castelo-Branco MT, Carvalho AT, Margallo VS, Delgado CF, Dines I and Elia CC: Apoptosis in the intestinal mucosa of patients with inflammatory bowel disease: Evidence of altered expression of FasL and perforin cytotoxic pathways. Int J Colorectal Dis. 20:277–286. 2005. View Article : Google Scholar : PubMed/NCBI
25	Myers BS, Martin JS, Dempsey DT, Parkman HP, Thomas RM and Ryan JP: Acute experimental colitis decreases colonic circular smooth muscle contractility in rats. Am J Physiol. 273:G928–G936. 1997.PubMed/NCBI
26	Pedersen J, LaCasse EC, Seidelin JB, Coskun M and Nielsen OH: Inhibitors of apoptosis (IAPs) regulate intestinal immunity and inflammatory bowel disease (IBD) inflammation. Trends Mol Med. 20:652–665. 2014. View Article : Google Scholar : PubMed/NCBI
27	Bhattacharyya A, Chattopadhyay R, Mitra S and Crowe SE: Oxidative stress: An essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 94:329–354. 2014. View Article : Google Scholar : PubMed/NCBI
28	Becker C, Watson AJ and Neurath MF: Complex roles of caspases in the pathogenesis of inflammatory bowel disease. Gastroenterology. 144:283–293. 2013. View Article : Google Scholar : PubMed/NCBI
29	Weimann BI and Hermann D: Studies on wound healing: Effects of calcium D-pantothenate on the migration, proliferation and protein synthesis of human dermal fibroblasts in culture. Int J Vitam Nutr Res. 69:113–119. 1999. View Article : Google Scholar : PubMed/NCBI
30	Halliwell B and Chirico S: Lipid peroxidation: Its mechanism, measurement, and significance. Am J Clin Nutr. 57(5 Suppl): 715S–724S, 725S. 1993.PubMed/NCBI
31	Kaya E, Gür ES, Ozgüç H, Bayer A and Tokyay R: L-glutamine enemas attenuate mucosal injury in experimental colitis. Dis Colon Rectum. 42:1209–1215. 1999. View Article : Google Scholar : PubMed/NCBI
32	Southorn P and Powıs G: Free radicals in medicine. I. Chemical nature and biologic reactions. Mayo Clin Proc. 63:381–389. 1988. View Article : Google Scholar : PubMed/NCBI
33	Cetinkaya A, Bulbuloglu E, Kurutas EB, Ciralik H, Kantarceken B and Buyukbese MA: Beneficial effects of N-acetylcysteine on acetic acid-induced colitis in rats. Tohoku J Exp Med. 206:131–139. 2005. View Article : Google Scholar : PubMed/NCBI
34	Alzoghaibi MA, Al Mofleh IA and Al-Jebreen AM: Lipid peroxides in patients with inflammatory bowel disease. Saudi J Gastroenterol. 13:187–190. 2007. View Article : Google Scholar : PubMed/NCBI
35	Byrav DSP, Medhi B, Prakash A, Chakrabarti A, Vaiphei K and Khanduja KL: Comparative evaluation of different doses of PPAR-γ agonist alone and in combination with sulfasalazine in experimentally induced inflammatory bowel disease in rats. Pharmacol Rep. 65:951–959. 2013. View Article : Google Scholar : PubMed/NCBI
36	Gul M, Kayhan B, Elbe H, Dogan Z and Otlu A: Histological and biochemical effects of dexmedetomidine on liver during an inflammatory bowel disease. Ultrastruct Pathol. 39:6–12. 2015. View Article : Google Scholar : PubMed/NCBI
37	Thomas MJ: The role of free radicals and antioxidants: How do we know that they are working? Crit Rev Food Sci Nutr. 35:21–39. 1995. View Article : Google Scholar : PubMed/NCBI
38	Flora G, Gupta D and Tiwari A: Toxicity of lead: A review with recent updates. Interdiscip Toxicol. 5:47–58. 2012. View Article : Google Scholar : PubMed/NCBI
39	Krzystek-Korpacka M, Neubauer K, Berdowska I, Zielinski B, Paradowski L and Gamian A: Impaired erythrocyte antioxidant defense in active inflammatory bowel disease: Impact of anemia and treatment. Inflamm Bowel Dis. 16:1467–1475. 2010. View Article : Google Scholar : PubMed/NCBI
40	D'Odorico A, Bortolan S, Cardin R, D'Inca' R, Martines D, Ferronato A and Sturniolo GC: Reduced plasma antioxidant concentrations and increased oxidative DNA damage in inflammatory bowel disease. Scand J Gastroenterol. 36:1289–1294. 2001. View Article : Google Scholar : PubMed/NCBI
41	Kruidenier L, Kuiper I, van Duijn W, Marklund SL, van Hogezand RA, Lamers CB and Verspaget HW: Differential mucosal expression of three superoxide dismutase isoforms in inflammatory bowel disease. J Pathol. 201:7–16. 2003. View Article : Google Scholar : PubMed/NCBI
42	Nieto N, Torres MI, Fernández MI, Girón MD, Ríos A, Suárez MD and Gil A: Experimental ulcerative colitis impairs antioxidant defense system in rat intestine. Dig Dis Sci. 45:1820–1827. 2000. View Article : Google Scholar : PubMed/NCBI
43	El-Beltagi HS and Mohamed HI: Reactive oxygen species, lipid peroxidation and antioxidative defense mechanism. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 41:44–57. 2013.
44	Hagar HH, El-Medany A, El-Eter E and Arafa M: Ameliorative effect of pyrrolidinedithiocarbamate on acetic acid induced colitis in rats. Eur J Pharmacol. 554:69–77. 2007. View Article : Google Scholar : PubMed/NCBI
45	Ermis H, Parlakpinar H, Gulbas G, Vardi N, Polat A, Cetin A, Kilic T and Aytemur ZA: Protective effect of dexpanthenol on bleomycin-induced pulmonary fibrosis in rats. Naunyn Schmiedebergs Arch Pharmacol. 386:1103–1110. 2013. View Article : Google Scholar : PubMed/NCBI