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Article

Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes

  • Authors:
    • Shu‑Guang Gao
    • Yang Yu
    • Chao Zeng
    • Shi‑Tao Lu
    • Jian Tian
    • Chao Cheng
    • Liang‑Jun Li
    • Guang‑Hua Lei
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 3488-3494
    |
    Published online on: October 5, 2016
       https://doi.org/10.3892/etm.2016.3784
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Abstract

The aim of the present study was to investigate the effects of phosphorylated osteopontin (p-OPN) on apoptosis and pro-inflammatory cytokine expression in human knee osteoarthritis (OA) chondrocytes. Human knee OA chondrocytes obtained from patients who underwent total knee arthroplasty were treated with p‑OPN, OPN or buffer. Reverse transcription quantitative‑polymerase chain reaction (RT‑qPCR) and western blot analysis were used to assess the expression levels of proinflammatory factors, including interleukin (IL)‑1β, tumor necrosis factor (TNF)‑α, IL‑6 and nuclear factor (NF)‑κB. Apoptosis of human knee OA chondrocytes was detected by Annexin V-fluorescein isothiocyanate/propidium iodide flow cytometry. Compared with the controls, chondrocytes treated with OPN exhibited higher mRNA and protein expression levels of proinflammatory factors (IL‑1β, TNF‑α, IL‑6 and NF‑κB), and a higher percentage of apoptotic chondrocytes. Furthermore, chondrocytes treated with p‑OPN exhibited the highest mRNA and protein expression levels of proinflammatory factors (IL‑1β, TNF‑α, IL‑6, NF‑κB) and the highest percentage of apoptotic chondrocytes. p‑OPN induces chondrocyte apoptosis and proinflammatory factor release, which suggests that p‑OPN may contribute to OA pathogenesis, and inhibition of p‑OPN may provide a novel effective strategy to slow or halt OA progression.
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Copy and paste a formatted citation
Spandidos Publications style
Gao SG, Yu Y, Zeng C, Lu ST, Tian J, Cheng C, Li LJ and Lei GH: Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med 12: 3488-3494, 2016.
APA
Gao, S., Yu, Y., Zeng, C., Lu, S., Tian, J., Cheng, C. ... Lei, G. (2016). Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Experimental and Therapeutic Medicine, 12, 3488-3494. https://doi.org/10.3892/etm.2016.3784
MLA
Gao, S., Yu, Y., Zeng, C., Lu, S., Tian, J., Cheng, C., Li, L., Lei, G."Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes". Experimental and Therapeutic Medicine 12.5 (2016): 3488-3494.
Chicago
Gao, S., Yu, Y., Zeng, C., Lu, S., Tian, J., Cheng, C., Li, L., Lei, G."Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3488-3494. https://doi.org/10.3892/etm.2016.3784
Copy and paste a formatted citation
x
Spandidos Publications style
Gao SG, Yu Y, Zeng C, Lu ST, Tian J, Cheng C, Li LJ and Lei GH: Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med 12: 3488-3494, 2016.
APA
Gao, S., Yu, Y., Zeng, C., Lu, S., Tian, J., Cheng, C. ... Lei, G. (2016). Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Experimental and Therapeutic Medicine, 12, 3488-3494. https://doi.org/10.3892/etm.2016.3784
MLA
Gao, S., Yu, Y., Zeng, C., Lu, S., Tian, J., Cheng, C., Li, L., Lei, G."Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes". Experimental and Therapeutic Medicine 12.5 (2016): 3488-3494.
Chicago
Gao, S., Yu, Y., Zeng, C., Lu, S., Tian, J., Cheng, C., Li, L., Lei, G."Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3488-3494. https://doi.org/10.3892/etm.2016.3784
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