Open Access

Role of peptidylarginine deiminase type 4 in gastric cancer

  • Authors:
    • Jiang Xin
    • Xiuqi Song
  • View Affiliations

  • Published online on: October 11, 2016     https://doi.org/10.3892/etm.2016.3798
  • Pages: 3155-3160
  • Copyright: © Xin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Peptidylarginine deiminase type 4 (PADI4) post-translationally converts peptidylarginine to citrulline, appearing to be overexpressed in numerous carcinomas. The current study aimed to investigate the expression of PADI4 in gastric cancer tissues and its effect on the biological activities of SGC‑7901 and AGS tumor cell lines. The expression of PADI4 was determined in gastric cancer and normal gastric mucosa tissues using western blot analysis and reverse transcription‑quantitative polymerase chain reaction. Gastric cancer cell lines were divided into the following groups: Mock group (subjected to transfection reagent); negative group [subjected to small interfering RNA (siRNA) transfection]; PADI4 siRNA group (subjected to PADI4 siRNA transfection); 5‑fluorouracil (5‑Fu) group (subjected to 5‑Fu); and 5‑Fu + siRNA transfection group (subjected to 5‑Fu and PADI4 siRNA transfection). The effects of silencing PADI4 with the above measures on the proliferation and invasion of SGC‑7901 and AGS cells were determined by MTT and Transwell chamber assays. In addition, propidium iodide staining was performed to detect the effects of PADI4 on the cell cycle. A significant increase in the expression of PADI4 mRNA in gastric cancer tissue compared with normal mucosa tissue was identified (P<0.05). The proliferation and invasion of SGC‑7901 and AGS cells were significantly decreased in the PADI4 siRNA group. Furthermore, flow cytometry DNA analysis revealed that silencing PADI4 resulted in significant S phase arrest and marked decrease of cells in the G2/M phase. PADI4 siRNA coupled with 5‑Fu significantly enhanced its inhibitory effect on the proliferation of gastric cancer cells. In conclusion, PADI4 demonstrated high expression in gastric cancer and served an important role in the biological activities of gastric cancer cells involving cell proliferation, invasion and cell cycle. As a result, PADI4 may be a valid cancer susceptibility gene and potential target for cancer therapy.
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November-2016
Volume 12 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Xin J and Song X: Role of peptidylarginine deiminase type 4 in gastric cancer. Exp Ther Med 12: 3155-3160, 2016.
APA
Xin, J., & Song, X. (2016). Role of peptidylarginine deiminase type 4 in gastric cancer. Experimental and Therapeutic Medicine, 12, 3155-3160. https://doi.org/10.3892/etm.2016.3798
MLA
Xin, J., Song, X."Role of peptidylarginine deiminase type 4 in gastric cancer". Experimental and Therapeutic Medicine 12.5 (2016): 3155-3160.
Chicago
Xin, J., Song, X."Role of peptidylarginine deiminase type 4 in gastric cancer". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3155-3160. https://doi.org/10.3892/etm.2016.3798