Oxidized low-density lipoprotein decreases VEGFR2 expression in HUVECs and impairs angiogenesis

  • Authors:
    • Min Zhang
    • Li Jiang
  • View Affiliations

  • Published online on: October 19, 2016     https://doi.org/10.3892/etm.2016.3823
  • Pages: 3742-3748
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Abstract

Atherosclerosis (AS), which is triggered by endothelial cell injury, evolves into a chronic inflammatory disease. Oxidized low‑density lipoprotein (ox‑LDL) is an important risk factor for the development of atherosclerosis; ox‑LDL induces atherosclerotic plaque formation via scavenging receptors. The present study used ox‑LDL‑treated human umbilical vein endothelial cells (HUVECs) to investigate the effect of ox‑LDL on angiogenesis. ox‑LDL decreased HUVEC proliferation by MTT, induced apoptosis by Annexin V‑fluorescein isothiocyanate (FITC) staining and markedly suppressed HUVEC tube formation by the Matrigel assay in a dose‑dependent manner. Angiogenesis has been correlated with monocyte invasion, plaque instability and atherosclerotic lesion formation. In addition, ox‑LDL induced the overproduction of reactive oxygen species using DCFH‑DA staining and increased caspase‑3 activity. Vascular endothelial growth factor receptor 2 (VEGFR2) were detected by quantitative polymerase chain reaction and western blot analysis and has previously been observed to have a key role in angiogenesis. Furthermore, the present study demonstrated that the abundance of VEGFR2 was decreased in ox‑LDL‑treated HUVECs. These results suggested that ox‑LDL impairs angiogenesis via VEGFR2 degradation, thus suggesting that VEGFR2 may be involved in adaptation to oxidative stress and AS.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang M and Jiang L: Oxidized low-density lipoprotein decreases VEGFR2 expression in HUVECs and impairs angiogenesis. Exp Ther Med 12: 3742-3748, 2016.
APA
Zhang, M., & Jiang, L. (2016). Oxidized low-density lipoprotein decreases VEGFR2 expression in HUVECs and impairs angiogenesis. Experimental and Therapeutic Medicine, 12, 3742-3748. https://doi.org/10.3892/etm.2016.3823
MLA
Zhang, M., Jiang, L."Oxidized low-density lipoprotein decreases VEGFR2 expression in HUVECs and impairs angiogenesis". Experimental and Therapeutic Medicine 12.6 (2016): 3742-3748.
Chicago
Zhang, M., Jiang, L."Oxidized low-density lipoprotein decreases VEGFR2 expression in HUVECs and impairs angiogenesis". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3742-3748. https://doi.org/10.3892/etm.2016.3823