Role of miR-145 in chronic constriction injury in rats

  • Authors:
    • Xiaolin Pang
    • Yuanzhang Tang
    • Dongya Zhang
  • View Affiliations

  • Published online on: November 14, 2016     https://doi.org/10.3892/etm.2016.3900
  • Pages: 4121-4127
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Abstract

The present study aims to investigate the effects and underlying mechanisms of miRNA-145 (miR-145) in rat models of chronic constriction injury (CCI). Rats were randomly divided into control, sham, CCI, agomiRNA (agomiR)‑normal control (NC) and agomiR-145 groups (n=25 in each group); in addition, 30 rats with CCI were divided into small hairpin (sh)RNA‑NC and shRNA‑ras responsive element binding protein 1 (RREB1) groups. Paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT) were detected. Reverse transcription‑quantitative polymerase chain reaction was used to detect miR‑145 expression levels, and western blotting was performed to measure RREB1 and phosphorylated-protein kinase B (p‑AKT) expression levels. In addition, a dual luciferase reporter assay was conducted to identify the target gene of miR‑145. PWMT and PWTL were decreased in CCI rats and this decrease was alleviated by miR‑145 injection. At 1, 3, 5 and 7 days after CCI, miR‑145 expression level in the spinal cord tissue of rats in the CCI group was significantly decreased compared with 1 day before CCI (P<0.05). Compared with the CCI group, miR‑145 expression level in the agomiR‑145 group was significantly higher (P<0.05). In addition, expression levels of RREB1 and p-AKT were significantly increased in the CCI group and significantly decreased in the agomiR‑145 group (P<0.05). Furthermore, knockdown of RREB1 expression by shRNA‑RREB1 significantly increased values of PWMT and PWTL, decreased expression levels of RREB1 and p‑AKT, and increased miR‑145 expression levels (P<0.05). Further investigation demonstrated that miR‑145 can bind with RREB1 mRNA. In conclusion, miR‑145 may be involved in the development of CCI through regulating the expression of RREB1.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Pang X, Tang Y and Zhang D: Role of miR-145 in chronic constriction injury in rats. Exp Ther Med 12: 4121-4127, 2016.
APA
Pang, X., Tang, Y., & Zhang, D. (2016). Role of miR-145 in chronic constriction injury in rats. Experimental and Therapeutic Medicine, 12, 4121-4127. https://doi.org/10.3892/etm.2016.3900
MLA
Pang, X., Tang, Y., Zhang, D."Role of miR-145 in chronic constriction injury in rats". Experimental and Therapeutic Medicine 12.6 (2016): 4121-4127.
Chicago
Pang, X., Tang, Y., Zhang, D."Role of miR-145 in chronic constriction injury in rats". Experimental and Therapeutic Medicine 12, no. 6 (2016): 4121-4127. https://doi.org/10.3892/etm.2016.3900