MicroRNA-376b promotes breast cancer metastasis by targeting Hoxd10 directly

An,Ning; Luo,Xinmei; Zhang,Ming; Yu,Ruilian

doi:10.3892/etm.2016.3942

MicroRNA-376b promotes breast cancer metastasis by targeting Hoxd10 directly

Authors:
- Ning An
- Xinmei Luo
- Ming Zhang
- Ruilian Yu
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Affiliations: Department of Oncology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: December 1, 2016 https://doi.org/10.3892/etm.2016.3942
Pages: 79-84
Copyright: © An et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Breast cancer is the most common malignant disease in women, and metastasis formed at distant anatomic sites was the major cause of cancer-related mortality. Thus, a novel therapy target and progression biomarker for breast cancer metastasis was necessary. microRNA (miR)‑376b has been demonstrated to regulate angiogenesis; however, its role in cancer metastasis remains elusive. In the present study, the expression of miR‑376b in normal breast tissue, JC and 4T1 cells was determined by qPCR. Furthermore, in vitro and in vivo experiments were performed to determine the effect of miR-376b on breast cancer metastasis. The direct target of miR‑376b was determined by the luciferase assay and western blotting. The results indicated that silencing of miR‑376b by the miR‑376‑mimic significantly inhibited 4T1 cell migration and invasion in vitro. Lung metastasis was also evidently decreased after silencing of miR‑376b in 4T1 cells. Moreover, the luciferase assay and western blotting identified that Hoxd10 is the direct target of miR‑376b during the regulation of breast cancer metastasis. To the best of our knowledge, the present study was the first to demonstrate the promoting breast cancer metastasis role of miR‑376b by directly targeting Hoxd10. Therefore, it would be a novel therapy target and prognostic biomarker for breast cancer.

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