Open Access

Correlative study on the JAK-STAT/PSMβ3 signal transduction pathway in asthenozoospermia

  • Authors:
    • Junguo Li
    • Li Zhang
    • Bing Li
  • View Affiliations

  • Published online on: December 6, 2016     https://doi.org/10.3892/etm.2016.3959
  • Pages: 127-130
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the possible mechanism of Janus kinase (JAK)-signal transduction and activator of transcription (STAT)/PSMβ3 signaling in the occurrence of asthenozoospermia. We examined seminal fluid samples from 30 cases of asthenozoospermia and 30 healthy controls. Sperm was collected using the Percoll density gradient centrifugation method. The expression of JAK, STAT and PSMβ3 mRNA was assessed by reverse-transcription quantitative PCR and the protein levels of p-JAK, p-STAT and PSMβ3 were measured by western blot analysis. The PSMβ3 mRNA and protein expression levels were also measured after application of a JAK inhibitor, AG-490, to the control group, with a FITC-labeled monoclonal rabbit anti‑human PSMβ3 primary antibody. The cells were observed under a laser confocal microscope. The mRNA levels of JAK, STAT and PSMβ3 in asthenozoospermia were decreased significantly (P<0.05). The protein levels of p-JAK, p-STAT and PSMβ3 in asthenozoospermia were also reduced and the differences were statistically significant (P<0.05). The PSMβ3 mRNA and protein expression levels were decreased in the control group after treatment with the JAK inhibitor, and levels were approximately equal to those of the asthenozoospermia group. PSMβ3 was mainly expressed in round-headed sperm, and less in asthenozoospermia. In conclusion, the JAK-STAT/PSMβ3 signaling transduction pathway may be involved in the pathogenic mechanism of asthenozoospermia.
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January-2017
Volume 13 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li J, Zhang L and Li B: Correlative study on the JAK-STAT/PSMβ3 signal transduction pathway in asthenozoospermia. Exp Ther Med 13: 127-130, 2017.
APA
Li, J., Zhang, L., & Li, B. (2017). Correlative study on the JAK-STAT/PSMβ3 signal transduction pathway in asthenozoospermia. Experimental and Therapeutic Medicine, 13, 127-130. https://doi.org/10.3892/etm.2016.3959
MLA
Li, J., Zhang, L., Li, B."Correlative study on the JAK-STAT/PSMβ3 signal transduction pathway in asthenozoospermia". Experimental and Therapeutic Medicine 13.1 (2017): 127-130.
Chicago
Li, J., Zhang, L., Li, B."Correlative study on the JAK-STAT/PSMβ3 signal transduction pathway in asthenozoospermia". Experimental and Therapeutic Medicine 13, no. 1 (2017): 127-130. https://doi.org/10.3892/etm.2016.3959