Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation ability

  • Authors:
    • Hidemi Hattori
    • Masayuki Ishihara
  • View Affiliations

  • Published online on: January 13, 2017     https://doi.org/10.3892/etm.2017.4041
  • Pages: 1176-1180
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Abstract

Platelet-rich plasma (PRP) is blood plasma containing a high number of platelets that release growth factors for wound healing and tissue regeneration. In the present study, the feasibility of improving PRP therapy by using chitosan that exhibits high platelet activation ability was investigated. A total of 13 chitosan samples with different molecular weight (Mw) and degree of deacetylation (DDA) were individually added to blood samples of rats and the amount of growth factors, albumin and fibrinogen in plasma was measured. To examine the influence of plasma activated by chitosan on the proliferation of fibroblasts and adipose tissue‑derived stromal cells (ASCs), the plasma was added to the culture medium of human fibroblasts and adipose tissue‑derived stromal cells. Chitosan with a DDA of >75% increased the release of platelet factor 4 into the plasma. The amount of growth factors released into the plasma and platelet activation varied depending on the Mw and DDA, while albumin and fibrinogen were hardly affected. The proliferation rate was highest when using plasma activated by chitosan with a DDA of 75‑85% and an Mw of 50,000‑190,000 Da. These results suggested that the effectiveness of PRP therapy may be improved by using chitosan with a DDA of 75‑85% and an Mw of 50,000‑190,000 Da.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Hattori H and Ishihara M: Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation ability. Exp Ther Med 13: 1176-1180, 2017.
APA
Hattori, H., & Ishihara, M. (2017). Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation ability. Experimental and Therapeutic Medicine, 13, 1176-1180. https://doi.org/10.3892/etm.2017.4041
MLA
Hattori, H., Ishihara, M."Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation ability". Experimental and Therapeutic Medicine 13.3 (2017): 1176-1180.
Chicago
Hattori, H., Ishihara, M."Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation ability". Experimental and Therapeutic Medicine 13, no. 3 (2017): 1176-1180. https://doi.org/10.3892/etm.2017.4041