MicroRNA‑17‑5p mediates hypoxia‑induced autophagy and inhibits apoptosis by targeting signal transducer and activator of transcription 3 in vascular smooth muscle cells

Hao,Ming‑Xiu; Wang,Xing; Jiao,Kun‑Li

doi:10.3892/etm.2017.4048

MicroRNA‑17‑5p mediates hypoxia‑induced autophagy and inhibits apoptosis by targeting signal transducer and activator of transcription 3 in vascular smooth muscle cells

Authors:
- Ming‑Xiu Hao
- Xing Wang
- Kun‑Li Jiao
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Affiliations: Department of Geriatrics, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China, Department of Endocrinology, Shanghai Pudong New Area Gongli Hospital, Second Military Medical University, Shanghai 200135, P.R. China, Department of Cardiology, Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, P.R. China
Published online on: January 16, 2017 https://doi.org/10.3892/etm.2017.4048
Pages: 935-941
Copyright: © Hao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate hypoxia-induced apoptosis and autophagy in vascular smooth muscle cells (VSMCs) and the underlying molecular mechanisms of microRNA (miR)‑17‑5p responses in an anaerobic environment. The results revealed that miR-17-5p expression was significantly upregulated in VSMCs subjected to hypoxic conditions (P<0.05) and lower miR‑17‑5p levels were observed in ethyl 3,4‑dihydroxybenzoate‑treated and hypoxia inducible factor‑1 loss‑of‑function cells. Additionally, it was demonstrated that miR‑17‑5p is associated with hypoxia‑induced autophagy, which was confirmed by upregulating the light chain 3‑II/LC3‑I ratio and downregulating nucleoporin p62. Cell apoptosis was inhibited in response to hypoxia, and levels of pro‑apoptotic proteins B‑cell lymphoma 2‑associated X protein and p‑caspase were markedly decreased when VSMCs were subjected to hypoxic conditions. Furthermore, expression of signal transducer and activator of transcription 3 (STAT3) decreased when cells were transfected with overexpressing miR‑17‑5p and subjected to hypoxic conditions, and the combination of miR‑17‑5p loss‑of‑function and hypoxia induced greater upregulation in the protein expression of STAT3 compared with a single treatment for hypoxia in VSMCs. In conclusion, miR‑17‑5p may be a novel hypoxia‑responsive miR and hypoxia may induce protective autophagy and anti‑apoptosis in VSMCs by targeting STAT3.

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