Downregulation of a novel long non‑coding RNA, LOC389332, is associated with poor prognosis and tumor progression in clear cell renal cell carcinoma

Jin,Ping; Wang,Juan; Liu,Yu

doi:10.3892/etm.2017.4080

Downregulation of a novel long non‑coding RNA, LOC389332, is associated with poor prognosis and tumor progression in clear cell renal cell carcinoma

Authors:
- Ping Jin
- Juan Wang
- Yu Liu
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Affiliations: Department of Pharmacy, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Clinical Laboratory, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Blood Transfusion, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
Published online on: January 24, 2017 https://doi.org/10.3892/etm.2017.4080
Pages: 1137-1142

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Abstract

It has been demonstrated that various long non‑coding RNAs (lncRNAs) may have key roles in various types of cancer. Clear cell renal cell carcinoma (ccRCC) is the most common subtype of all RCCs, accounting for 70‑80% of all cases. The present study identified a novel lncRNA and investigated its clinical significance and physiological function in ccRCC. The expression pattern of the novel lncRNA LOC389332 in 30 ccRCC tissue samples was examined using reverse‑transcription quantitative polymerase chain reaction. The results demonstrated that LOC389332 expression was markedly lower in ccRCC tissues compared with that in matched adjacent non‑tumor tissues. Of note, downregulation of LOC389332 expression was significantly associated with the tumor American Joint Commission on Cancer stage (P=0.001), Fuhrman grade (P=0.001) and lymph node metastasis (P<0.001). Furthermore, patients with ccRCC with lower levels of LOC389332 expression had a shorter overall survival time than those with higher LOC389332 expression. A gain‑of‑function study was used to evaluate the biological function of LOC389332 in ccRCC and the results suggested that restoration of LOC389332 expression inhibited the growth and migration of the 786‑O and 769‑P cell lines. Therefore, the results of the present study demonstrated that LOC389332 is a novel lncRNA involved in ccRCC progression and may be a potential diagnostic and prognostic biomarker. Ectopic overexpression of LOC389332 may represent a therapeutic strategy for ccRCC.

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