Involvement of neuronal pathways in the protective effects of hindlimb perconditioning during renal ischemia

Sedaghat,Zahra; Kadkhodaee,Mehri; Seifi,Behjat; Ahghari,Parisa; Pourkhalili,Khalil; Akbari,Zahra; Sadeghi,Mehdi

doi:10.3892/etm.2017.4175

Involvement of neuronal pathways in the protective effects of hindlimb perconditioning during renal ischemia

Authors:
- Zahra Sedaghat
- Mehri Kadkhodaee
- Behjat Seifi
- Parisa Ahghari
- Khalil Pourkhalili
- Zahra Akbari
- Mehdi Sadeghi
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Affiliations: Department of Physiology, Bushehr University of Medical Sciences, School of Medicine, Bushehr 7514633341, Iran, Department of Physiology, Tehran University of Medical Sciences, School of Medicine, Tehran 1417613151, Iran, Department of Physiology, Tehran University of Medical Sciences (International Branch), School of Medicine, Tehran 1417613151, Iran
Published online on: March 2, 2017 https://doi.org/10.3892/etm.2017.4175
Pages: 1956-1960

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Abstract

Remote ischemic perconditioning (RPEC) is a therapeutic intervention that has been demonstrated to reduce renal ischemia/reperfusion (I/R) injury. However, the underlying renal protective mechanism remains unclear. The present study hypothesized that RPEC may utilize neural pathways to transfer the protective signal from the perconditioned hindlimb to the kidney. Following a right nephrectomy, rats were randomly allocated into five groups (n=6). The sham group underwent the surgical protocol only. In all other groups, the left renal pedicle was clamped for 45 min and reperfused for 24 h. The I/R control group then underwent 45 min ischemia and 24 h reperfusion (I/R) with no more intervention but the I/R‑NR control group underwent the ischemia and reperfusion followed by left femoral nerve (FN) and sciatic nerve (SN) resection. The RPEC group underwent ischemia and reperfusion followed by four cycles of 5 min occlusions of the left femoral artery and 5 min reperfusion. Finally, the RPEC‑NR group underwent ischemia and reperfusion followed by left FN and SN resection. Following 24 h, renal functional indices, plasma blood urea nitrogen (BUN) and creatinine (Cr) levels, urinary N‑acetyl‑β‑glucosaminidase (NAG) release and histopathological changes were assessed. Compared with the sham group, ischemia and reperfusion in the sham and I/R control groups resulted in renal dysfunction, indicated by significantly increased levels of BUN and Cr. This was accompanied by increased urinary NAG activity and morphological damage observed in control groups. In the RPEC group, renal histology and function were significantly improved compared with the control groups. However, FN and SN resection eliminated the protection of the kidney, which was induced by RPEC. In conclusion, remote hindlimb ischemic perconditioning reduced renal I/R injury in the rat kidney in a manner that potentially involves a neural pathway.

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