Amniotic membrane-derived stem cells help repair osteochondral defect in a weight-bearing area in rabbits

Zhang,Zhijin; Zeng,Linru; Yang,Jun; Guo,Lin; Hou,Qiao; Zhu,Fangbing

doi:10.3892/etm.2017.4497

Amniotic membrane-derived stem cells help repair osteochondral defect in a weight-bearing area in rabbits

Authors:
- Zhijin Zhang
- Linru Zeng
- Jun Yang
- Lin Guo
- Qiao Hou
- Fangbing Zhu
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Affiliations: Department of Hand Surgery, Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang 311201, P.R. China, Department of Foot and Ankle Surgery, Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang 311201, P.R. China, Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China, Department of Orthopaedics, Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang 311201, P.R. China
Published online on: May 23, 2017 https://doi.org/10.3892/etm.2017.4497
Pages: 187-192
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Our study evaluated the use of amniotic membrane-derived stem cells for repairing osteochondral defects in a weight-bearing area in rabbits. Twenty-four 3-month-old male or female New Zealand white rabbits were selected. The rabbits were randomly divided into 3 groups of eight, according to the treatment received for an experimentally inflicted femoral medial malleolus lesion, group I received a human acellular amniotic membrane seeded with bone marrow-derived mesenchymal stem cells (HAAM-BMSCs) implant; group II received a simple HAAM implant and the control group received no experimental lesion or treatment. The rabbits were sacrificed at 12 and 24 weeks after the procedures (4 rabbits in each time-point) and the cartilage repair status in each animal was evaluated under the microscope. The tissue of the HAAM-BMSCs group grew well covering an area in the visual field that was significantly larger than that of the HAAM group (p<0.05). The percentage of collagen II-positive area in the HAAM-BMSC group was significantly higher than that in HAAM group (p<0.05). The number of chondrocytes determined by toluidine blue staining was higher in the HAAM-BMSC group than that in the HAAM group (p<0.05). The Wakitani scores of the HAAM and HAAM-BMSC groups were significantly higher (worse) than those of the normal control group (p<0.05), but the score in the HAAM-BMSC group was significantly lower than that in the HAAM group (p<0.05). The Wakitani scores in the HAAM-BMSC group were not different between the two time-points taken. Based on our findings, the amniotic membrane-derived stem cells had a good therapeutic effect in repairing the osteochondral defects in the weight-bearing area, and the number of chondrocytes in the injured area was increased significantly, which accelerated the repair of the damaged tissue in rabbits.

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