Anti-CXCL4 monoclonal antibody accelerates telogen to anagen transition and attenuates apoptosis of the hair follicle in mice

Guan,Wen; Yu,Xiaolan; Li,Jingjing; Deng,Qing; Zhang,Yang; Gao,Jing; Xia,Peng; Yuan,Yunsheng; Gao,Jin; Zhou,Liang; Han,Wei; Yu,Yan

doi:10.3892/etm.2017.4578

Anti-CXCL4 monoclonal antibody accelerates telogen to anagen transition and attenuates apoptosis of the hair follicle in mice

Authors:
- Wen Guan
- Xiaolan Yu
- Jingjing Li
- Qing Deng
- Yang Zhang
- Jing Gao
- Peng Xia
- Yunsheng Yuan
- Jin Gao
- Liang Zhou
- Wei Han
- Yan Yu
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Affiliations: Shanghai Municipality Key Laboratory of Veterinary Biotechnology, Department of Animal Sciences, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, P.R. China, Laboratory of Regeneromics, School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, P.R. China
Published online on: June 12, 2017 https://doi.org/10.3892/etm.2017.4578
Pages: 1001-1008
Copyright: © Guan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Although hair loss or alopecia is a common disease, its exact mechanisms are not yet well understood. The present study investigated the hypothesis that the homeostatic regulation of genes during hair regeneration may participate in hair loss, based on the cyclicity of hair growth. A cluster of such genes was identified by an expression gene‑array from the dorsal skin in a depilated mouse model, and CXCL4 was identified as a significantly regulated gene during the hair regeneration process. To elucidate the function of CXCL4 in hair growth, CXCL4 activity was blocked by the administration of an anti‑CXCL4 monoclonal antibody (mAb). Histomorphometric analysis indicated that anti‑CXCL4 mAb induced an earlier anagen phase and delayed hair follicle regression, in contrast with that in the control group. Moreover, CXCL4 mAb upregulated the transcription levels of several hair growth‑related genes, including Lef1, Wnt10b, Bmp4 and Bmp2. In addition, CXCL4 mAb increased the levels of the proliferation‑related protein PCNA and Bcl‑2 during the anagen phase, while it reduced the expression of pro‑apoptotic protein Bax and cleaved caspase‑3 during the catagen phase. These findings reveal that CXCL4 plays an important role in hair growth, and that blockade of CXCL4 activity promotes hair growth.

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