Effects of low dose of ethanol on the senescence score, brain function and gene expression in senescence‑accelerated mice 8 (SAMP8)

Kimoto,Akiko; Izu,Hanae; Fu,Churan; Suidasari,Sofya; Kato,Norihisa

doi:10.3892/etm.2017.4633

Effects of low dose of ethanol on the senescence score, brain function and gene expression in senescence‑accelerated mice 8 (SAMP8)

Authors:
- Akiko Kimoto
- Hanae Izu
- Churan Fu
- Sofya Suidasari
- Norihisa Kato
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Affiliations: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Higashi‑Hiroshima, Hiroshima 739‑8528, Japan, Quality and Evaluation Research Division, National Research Institute of Brewing, Higashi‑Hiroshima, Hiroshima 739‑0046, Japan
Published online on: June 20, 2017 https://doi.org/10.3892/etm.2017.4633
Pages: 1433-1440
Copyright: © Kimoto et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Accumulating epidemiological evidence suggests light to moderate alcohol intake reduces risk of several chronic diseases. However, there is limited information regarding the effects of low alcohol intake in animal studies. This study investigated the effect of low ethanol dosage on senescence‑accelerated mouse (SAMP8), an animal model of aging and neurodegenaration. Male SAMP8 mice (11 weeks old) had free access to a commercial stock diet with drinking water containing 0, 1 or 2% (v/v) ethanol for 15 weeks. The total grading score of senescence in the 1%‑ethanol group was, in large part, the lowest among the three groups. Analysis using the open‑field test revealed a significant elevation (+77%, P<0.05) in the rearing activity (index of seeking behavior) in the 1%‑ethanol group, but not in the 2%‑ethanol group. In addition, 2% ethanol elevated spontaneous locomotor activity (+75%, P<0.05), whereas 1% ethanol did not. Scrutiny of serum parameters indicated intake of 1% ethanol significantly decreased serum insulin levels (‑13%, P<0.05), whereas 2% did not. Intake of 2% ethanol significantly elevated (2.5‑fold, P<0.05) S100a8 mRNA (an inflammatory signal) in the brain, but that of 1% ethanol did not. Intriguingly, 1% ethanol intake remarkably elevated (10‑fold, P<0.05) mRNA of brain alcohol dehydrogenase 1 (Adh1), which metabolizes lipid‑peroxidation products and is involved in the synthesis of retinoic acid, a neuroprotective factor. Of note, 2%‑ethanol intake did not exert this effect. Taken together, intake of 1% ethanol is likely to be beneficial for SAMP8 mice.

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