Open Access

Comparative study of dendritic cells matured by using IL‑1β, IL‑6, TNF‑α and prostaglandins E2 for different time span

  • Authors:
    • Xuejie Wu
    • Feng Xu
    • Jinliang Liu
    • Guiqiang Wang
  • View Affiliations

  • Published online on: June 22, 2017     https://doi.org/10.3892/etm.2017.4649
  • Pages: 1389-1394
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Interleukin (IL)‑1β, IL‑6, tumor necrosis factor (TNF)‑α and prostaglandins E2 is considered as the standard cocktail for maturing dendritic cells (DCs). However, the appropriate time span for DC maturation with the standard cocktail remains unclear. The present study aimed to compare the differences between DCs matured with the standard cocktail for 24 and 48 h, respectively, and determine whether 24‑h stimulation was sufficient for DC maturation. The findings demonstrated that, compared with DCs matured for 48 h, the levels of cluster of differentiation (CD)80, CD83, CD86 and programmed death‑ligand 1 expression in DCs matured for 24 h were relatively lower. However, with the exception of CD80 whose mean fluorescence intensity (MFI) was higher in DCs matured for 48 h, the MFI values of other surface markers were comparable. Notably, the MFI of CD40 was higher in DCs matured for 24 h. In addition, the viability, T cell stimulatory capacity in allogeneic mixed lymphocyte reaction and cytokine production, including IL‑12p40, IL‑12p70 and IL‑10, were all comparable between DCs matured for 24 and 48 h, respectively. These results indicated that 24‑h stimulation may be sufficient for DC maturation when using the standard cocktail.
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August-2017
Volume 14 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wu X, Xu F, Liu J and Wang G: Comparative study of dendritic cells matured by using IL‑1β, IL‑6, TNF‑α and prostaglandins E2 for different time span. Exp Ther Med 14: 1389-1394, 2017.
APA
Wu, X., Xu, F., Liu, J., & Wang, G. (2017). Comparative study of dendritic cells matured by using IL‑1β, IL‑6, TNF‑α and prostaglandins E2 for different time span. Experimental and Therapeutic Medicine, 14, 1389-1394. https://doi.org/10.3892/etm.2017.4649
MLA
Wu, X., Xu, F., Liu, J., Wang, G."Comparative study of dendritic cells matured by using IL‑1β, IL‑6, TNF‑α and prostaglandins E2 for different time span". Experimental and Therapeutic Medicine 14.2 (2017): 1389-1394.
Chicago
Wu, X., Xu, F., Liu, J., Wang, G."Comparative study of dendritic cells matured by using IL‑1β, IL‑6, TNF‑α and prostaglandins E2 for different time span". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1389-1394. https://doi.org/10.3892/etm.2017.4649