Open Access

Differentially methylated regions in patients with rheumatic heart disease and secondary pulmonary arterial hypertension

  • Authors:
    • Dawei Zheng
    • Xiaoying Chen
    • Ni Li
    • Lebo Sun
    • Qingyun Zhou
    • Huoshun Shi
    • Guodong Xu
    • Jing Liu
    • Limin Xu
    • Shiwei Duan
    • Guofeng Shao
  • View Affiliations

  • Published online on: June 22, 2017     https://doi.org/10.3892/etm.2017.4652
  • Pages: 1367-1372
  • Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to identify differentially methylated regions (DMRs) in patients with rheumatic heart disease and secondary pulmonary arterial hypertension (RHD‑PAH). A genome‑wide DNA methylation assay was performed between 6 patients with RHD‑PAH and 6 healthy controls using an Illumina Infinium HumanMethylation450 BeadChip kit. The Limma software package was subsequently used to identify significant DMRs. A total of 40 hypome­thylated and 64 hypermethylated CpG sites were identified between the RHD‑PAH group and the control group. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes term and signaling pathway enrichment analyses revealed that the DMRs, mapped to the genes including protein kinase C α, protein kinase AMP‑activated non‑catalytic subunit γ2, sprouty related EVH1 domain containing 2 and LIF interleukin 6 family cytokine, were significantly enriched in the negative regulation of protein kinase/transferase activity and the positive regulation of protein amino acid phosphorylation/phosphate metabolic process. The identified DMRs may provide novel insights into the pathogenesis of RHD‑PAH.
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August-2017
Volume 14 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zheng D, Chen X, Li N, Sun L, Zhou Q, Shi H, Xu G, Liu J, Xu L, Duan S, Duan S, et al: Differentially methylated regions in patients with rheumatic heart disease and secondary pulmonary arterial hypertension. Exp Ther Med 14: 1367-1372, 2017
APA
Zheng, D., Chen, X., Li, N., Sun, L., Zhou, Q., Shi, H. ... Shao, G. (2017). Differentially methylated regions in patients with rheumatic heart disease and secondary pulmonary arterial hypertension. Experimental and Therapeutic Medicine, 14, 1367-1372. https://doi.org/10.3892/etm.2017.4652
MLA
Zheng, D., Chen, X., Li, N., Sun, L., Zhou, Q., Shi, H., Xu, G., Liu, J., Xu, L., Duan, S., Shao, G."Differentially methylated regions in patients with rheumatic heart disease and secondary pulmonary arterial hypertension". Experimental and Therapeutic Medicine 14.2 (2017): 1367-1372.
Chicago
Zheng, D., Chen, X., Li, N., Sun, L., Zhou, Q., Shi, H., Xu, G., Liu, J., Xu, L., Duan, S., Shao, G."Differentially methylated regions in patients with rheumatic heart disease and secondary pulmonary arterial hypertension". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1367-1372. https://doi.org/10.3892/etm.2017.4652