Open Access

High mobility group box 1 protein attenuates myocardial ischemia reperfusion injury via inhibition of the p38 mitogen‑activated protein kinase signaling pathway

  • Authors:
    • Yan‑Hong Zhou
    • Qian‑Feng Han
    • Lan‑Hua Wang
    • Tao Liu
    • Xiao‑Yan Meng
    • Lei Wu
    • Tai Li
    • Yue‑Ru Jiao
    • Heng‑Chen Yao
    • De‑Yong Zhang
  • View Affiliations

  • Published online on: June 22, 2017     https://doi.org/10.3892/etm.2017.4653
  • Pages: 1582-1588
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to determine the effects of high mobility group box 1 protein (HMGB1) on myocardial ischemia reperfusion (I/R) injury in rats following acute myocardial ischemia and investigate the underlying molecular mechanisms of these effects. Male Wistar rats were randomly divided into the following groups (n=10/group): Sham operation; I/R; HMGB50 (50 ng/kg HMGB1 before I/R); HMGB100 (100 ng/kg HMGB1 before I/R); and HMGB200 (200 ng/kg HMGB1 before I/R). Serum cardiac troponin I (cTnI), interleukin (IL)‑6 and tumor necrosis factor (TNF)‑α levels were subsequently measured. Myocardial levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were also determined. Myocardial infarction size (IS) was determined by 2,3,5‑triphenyltetrazolium chloride staining. Myocardial expression of hypoxia inducible factor (HIF)‑1α and phosphorylated p38 mitogen‑activated protein kinase (P‑p38 MAPK) protein was measured using western blotting. The results demonstrated that HMGB1 significantly decreased serum levels of cTnI, IL‑6 and TNF‑α and myocardial IS in I/R rats compared with the sham group (all P<0.05). HMGB1 also significantly decreased and increased myocardial levels of MDA and SOD, respectively (both P<0.05). HMGB1 significantly increased myocardial expression of HIF‑1α and decreased expression of P‑p38 MAPK following I/R (both P<0.05). These effects of HMGB1 occurred in a dose‑ dependent manner. The results of the current study indicate that the cardioprotective effects of intravenous HMGB1 are associated with increased myocardial expression of HIF‑1α via inhibition of P‑p38 MAPK expression, leading to inhibition of the P‑p38 MAPK signaling pathway.
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August-2017
Volume 14 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Zhou YH, Han QF, Wang LH, Liu T, Meng XY, Wu L, Li T, Jiao YR, Yao HC, Zhang DY, Zhang DY, et al: High mobility group box 1 protein attenuates myocardial ischemia reperfusion injury via inhibition of the p38 mitogen‑activated protein kinase signaling pathway. Exp Ther Med 14: 1582-1588, 2017.
APA
Zhou, Y., Han, Q., Wang, L., Liu, T., Meng, X., Wu, L. ... Zhang, D. (2017). High mobility group box 1 protein attenuates myocardial ischemia reperfusion injury via inhibition of the p38 mitogen‑activated protein kinase signaling pathway. Experimental and Therapeutic Medicine, 14, 1582-1588. https://doi.org/10.3892/etm.2017.4653
MLA
Zhou, Y., Han, Q., Wang, L., Liu, T., Meng, X., Wu, L., Li, T., Jiao, Y., Yao, H., Zhang, D."High mobility group box 1 protein attenuates myocardial ischemia reperfusion injury via inhibition of the p38 mitogen‑activated protein kinase signaling pathway". Experimental and Therapeutic Medicine 14.2 (2017): 1582-1588.
Chicago
Zhou, Y., Han, Q., Wang, L., Liu, T., Meng, X., Wu, L., Li, T., Jiao, Y., Yao, H., Zhang, D."High mobility group box 1 protein attenuates myocardial ischemia reperfusion injury via inhibition of the p38 mitogen‑activated protein kinase signaling pathway". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1582-1588. https://doi.org/10.3892/etm.2017.4653