Hypoxia‑inducible factor 1α participates in hypoxia‑induced epithelial‑mesenchymal transition via response gene to complement 32

Zhu,Liang; Zhao,Qiu

doi:10.3892/etm.2017.4665

Hypoxia‑inducible factor 1α participates in hypoxia‑induced epithelial‑mesenchymal transition via response gene to complement 32

Authors:
- Liang Zhu
- Qiu Zhao
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Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
Published online on: June 23, 2017 https://doi.org/10.3892/etm.2017.4665
Pages: 1825-1831

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Abstract

The aim of the present study was to explore the function of response gene to complement 32 (RGC-32) in hypoxia‑induced epithelial‑mesenchymal transition (EMT) in pancreatic cancer. Three kinds of hypoxia‑inducible factor 1α (HIF‑1α) small interfering (si)RNA were synthesized and the different effects on the expression of HIF‑1α were detected by western blotting. In human pancreatic cancer BxPC‑3 cells, HIF‑1α levels were diminished using siRNA transfection or HIF‑1α inhibitor pretreatment, and the expression levels of RGC‑32 and EMT‑associated proteins were analyzed using reverse transcription‑quantitative polymerase chain reaction and western blotting. Subsequently, the protein levels of epithelial marker, E‑cadherin, and mesenchymal marker, vimentin, were determined by western blotting. Results demonstrated that HIF‑1α‑Homo‑488 siRNA and HIF‑1α‑Homo‑1216 siRNA diminished the protein level of HIF‑1α. Compared with normoxia, hypoxia induced the levels of HIF‑1α, RGC‑32, N‑cadherin and vimentin, but suppressed the expression of E‑cadherin and cytokeratins. The inhibition of HIF‑1α by HIF‑1α‑Homo‑1216 siRNA transfection or HIF‑1α inhibitor repressed hypoxia‑induced HIF‑1α, RGC‑32, N‑cadherin and vimentin, but increased the expression of E‑cadherin and cytokeratins. When RGC‑32 was knocked down, hypoxia‑induced vimentin was suppressed; however, hypoxia‑suppressed N‑cadherin was released. In conclusion, the present results demonstrated that hypoxia induced the expression of HIF‑1α to activate the levels of RGC‑32, in turn to regulate the expression EMT‑associated proteins for EMT. These findings revealed the function of RGC‑32 in hypoxia‑induced EMT and may have identified a novel link between HIF‑1α and EMT for pancreatic cancer therapy.

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