Overexpressed eNOS upregulates SIRT1 expression and protects mouse pancreatic β cells from apoptosis

Hu,Tingting; Chen,Ye; Jiang,Qian; Lin,Jun; Li,Hewei; Wang,Ping; Feng,Leping

doi:10.3892/etm.2017.4669

Overexpressed eNOS upregulates SIRT1 expression and protects mouse pancreatic β cells from apoptosis

Authors:
- Tingting Hu
- Ye Chen
- Qian Jiang
- Jun Lin
- Hewei Li
- Ping Wang
- Leping Feng
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Affiliations: Department of Biotechnology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China, Graduate School of Guilin Medical University, Guilin, Guangxi 541004, P.R. China, Department of Stomatology, The First Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541004, P.R. China
Published online on: June 26, 2017 https://doi.org/10.3892/etm.2017.4669
Pages: 1727-1731

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Abstract

Loss of sirtuin 1 (SIRT1) activity may be associated with metabolic diseases, including diabetes. The aim of the present study was to investigate the potential effects of overexpressed endothelial nitric oxide synthase (eNOS) on cell proliferation and apoptosis with SIRT1 activation in the Min6 mouse pancreatic β cell line. A pcDNA3.0‑eNOS plasmid was constructed and transfected into Min6 cells for 24 h prior to harvesting. eNOS expression was validated and SIRT1 expression was detected following plasmid transfection using reverse transcription‑quantitative polymerase chain reaction and western blot analysis, which demonstrated that the expression levels of eNOS and SIRT1 were significantly upregulated. Furthermore, the cell proliferation and cell apoptosis of the Min6 cells were evaluated, using a cell counting kit‑8 assay and flow cytometry, respectively. The results suggested that overexpressed eNOS promoted cell proliferation and inhibited cell apoptosis in Min6 cells. The interaction between eNOS and SIRT1 was explored through co‑immunoprecipitation, and it found that there was a strong interaction between eNOS and SIRT1. In conclusion, overexpressed eNOS may induce SIRT1 activation, which is implied to play a protective role in Min6 cells, and eNOS may be a new therapeutic target for diseases such as type 2 diabetes.

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