Comparison of the roles of house dust mite allergens, ovalbumin and lipopolysaccharides in the sensitization of mice to establish a model of severe neutrophilic asthma

Jia,Aijun; Wang,Yueling; Sun,Wenjin; Xiao,Bing; Mu,Lin; Wei,Yan; Xu,Li; Peng,Cong; Zhang,Dongshan; Shen,Huahao; Xiang,Xudong

doi:10.3892/etm.2017.4776

Comparison of the roles of house dust mite allergens, ovalbumin and lipopolysaccharides in the sensitization of mice to establish a model of severe neutrophilic asthma

Authors:
- Aijun Jia
- Yueling Wang
- Wenjin Sun
- Bing Xiao
- Lin Mu
- Yan Wei
- Li Xu
- Cong Peng
- Dongshan Zhang
- Huahao Shen
- Xudong Xiang
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Affiliations: Department of Respiratory Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Anesthesiology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China, Department of Emergency, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China, Department of Respiratory Medicine, Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China, The Second Department of Thoracic Medicine, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410006, P.R. China, Department of Dermatology and Venereology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China, Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China
Published online on: July 11, 2017 https://doi.org/10.3892/etm.2017.4776
Pages: 2126-2134
Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Neutrophilic asthma (NA) is associated with a severe disease course and poor response to corticosteroids. The present study aimed to compare the effects of various concentrations of house dust mite (HDM) allergens, ovalbumin (OVA), the major egg allergen, and lipopolysaccharide (LPS) in combination on the onset of severe NA. Female C57BL/6 mice were grouped according to a random number table and intranasally sensitized with HDM/LPS/OVA extracts on days 0, 1 and 2 of the study. In group 1, mice received 50 µg HDM + 50 µg OVA + 15 µg LPS, mice in group 2 received 50 µg HDM + 100 µg OVA + 15 µg LPS, mice in group 3 received 100 µg HDM + 50 µg OVA + 15 µg LPS and those in group 4 received 100 µg HDM + 100 µg OVA + 15 µg LPS, while mice in the control group received saline only. The mice were then challenged by OVA solution with atomized excitation on days 14, 15, 18, 19 and 20 for 30 min each. Ethology, airway hyperresponsiveness (AHR), immune cell distributions in bronchoalveolar lavage fluid (BALF), and specific cytokines interleukin 17A (IL‑17A) and IL‑4 in serum were assessed. Histological examination of inflammation by hematoxylin and eosin staining and immunohistochemical assessment of neutrophils (NEU), eosinophils (EOS), IL‑17A and IL‑4 were also performed. Compared with the control group, the HDM/OVA/LPS‑sensitized groups 1‑4 had markedly increased BALF cells, serum interleukin IL‑17A and IL‑4, inflammatory cell infiltration, EOS as well as IL‑17A and IL‑4 by immunohistochemical staining (all P<0.05). Among the four HDM/OVA/LPS‑sensitized groups, mice of group 4 had higher AHR, a significantly higher total cell number, NEU and EOS in BALF as well as significantly higher NEU and NEU/EOS ratios according to immunohistochemical staining when compared to groups 1‑3 (P<0.05 for all). In conclusion, sensitization with 100 µg HDM + 100 µg OVA + 15 µg LPS successfully established a severe asthma model with a predominantly neutrophilic inflammatory phenotype.