Open Access

Pancreatic islet regeneration through PDX-1/Notch-1/Ngn3 signaling after gastric bypass surgery in db/db mice

  • Authors:
    • Tao Huang
    • Jun Fu
    • Zhijing Zhang
    • Yuhao Zhang
    • Yunjia Liang
    • Cuicui Ge
    • Xianju Qin
  • View Affiliations

  • Published online on: August 7, 2017     https://doi.org/10.3892/etm.2017.4896
  • Pages: 2831-2838
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In view of the compelling anti-diabetic effects of gastric bypass surgery (GBS) in the treatment of morbid obesity, it is important to clarify its enhancing effect on pancreatic islets, which is closely linked with diabetes remission in obese patients, as well as the underlying mechanisms. The present study evaluated the effects of GBS on glycemic control and other pancreatic changes in db/db mice. The db/db mice were divided into Control, Sham and GBS group. A significant improvement in fasting plasma glucose levels and glucose intolerance were observed post‑surgery. At 4 weeks after surgery, further noteworthy changes were observed in the GBS group, including improved islet structure (revealed by immunohistochemical analysis), enhanced insulin secretion, pancreatic hyperplasia and a marked increase in the ratio of β‑cells to non‑β endocrine cells. Furthermore, notable changes in the levels of Notch‑1, pancreatic and duodenal homeobox 1 (PDX‑1) and neurogenin 3 (Ngn3) were observed in the GBS group, indicating a potential role of Notch signaling in pancreatic islet regeneration after surgery. In addition, results obtained in PDX‑1 knockout (KO), Notch‑1 KO and Ngn3 KO mouse models with GBS suggested that elevated PDX‑1 resulted in the inhibition of Notch‑1, further facilitated Ngn3 and thus promoted pancreatic β‑cell regeneration after GBS. The present findings demonstrated that GBS in db/db mice resulted in pancreatic islet regeneration through the PDX‑1/Notch‑1/Ngn3 signaling pathway, which also reflected the important role of the gastrointestinal system in metabolism control.

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October-2017
Volume 14 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Huang T, Fu J, Zhang Z, Zhang Y, Liang Y, Ge C and Qin X: Pancreatic islet regeneration through PDX-1/Notch-1/Ngn3 signaling after gastric bypass surgery in db/db mice. Exp Ther Med 14: 2831-2838, 2017
APA
Huang, T., Fu, J., Zhang, Z., Zhang, Y., Liang, Y., Ge, C., & Qin, X. (2017). Pancreatic islet regeneration through PDX-1/Notch-1/Ngn3 signaling after gastric bypass surgery in db/db mice. Experimental and Therapeutic Medicine, 14, 2831-2838. https://doi.org/10.3892/etm.2017.4896
MLA
Huang, T., Fu, J., Zhang, Z., Zhang, Y., Liang, Y., Ge, C., Qin, X."Pancreatic islet regeneration through PDX-1/Notch-1/Ngn3 signaling after gastric bypass surgery in db/db mice". Experimental and Therapeutic Medicine 14.4 (2017): 2831-2838.
Chicago
Huang, T., Fu, J., Zhang, Z., Zhang, Y., Liang, Y., Ge, C., Qin, X."Pancreatic islet regeneration through PDX-1/Notch-1/Ngn3 signaling after gastric bypass surgery in db/db mice". Experimental and Therapeutic Medicine 14, no. 4 (2017): 2831-2838. https://doi.org/10.3892/etm.2017.4896