Co-culture with lung cancer A549 cells promotes the proliferation and migration of mesenchymal stem cells derived from bone marrow
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- Published online on: August 8, 2017 https://doi.org/10.3892/etm.2017.4909
- Pages: 2983-2991
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Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
The initiation and progression of various types of tumors, such as lung neoplasms, are driven by a population of cells with stem cell properties and their microenvironment. Bone marrow mesenchymal stem cells (BM‑MSCs) in long‑term in vitro culture may exhibit spontaneous changes in stem cell biological properties, including malignant transformations; however, the molecular mechanisms of this have not been fully elucidated. In the present study, a BM‑MSC and lung cancer A549 cell co‑culture system was utilized to investigate how the tumor microenvironment may spontaneously change the proliferation, migration and differentiation of BM‑MSCs. It was demonstrated that the lung cancer A549 microenvironment is able to induce changes in the cell morphology, proliferation, karyotype, cytoskeleton and migration ability of BM‑MSCs in vitro. Compared with the control group BM‑MSCs, the expression of Ras, phosphorylated-extracellular regulated protein kinases, nuclear factor‑κB, P62 and B‑cell lymphoma 2 (Bcl‑2) proteins in groups of co‑cultured BM‑MSCs increased significantly (P<0.05) and the expression of P53, Bcl‑2 associated X protein and caspase‑3 protein decreased significantly (P<0.05). The mechanisms responsible for the changes observed in BM‑MSCs may be related to abnormal expression of related genes in the ERK signaling pathway.
