Therapeutic effects of stemonine on particulate matter 2.5‑induced chronic obstructive pulmonary disease in mice

Zhang,Jinbo; Li,Shiqing; Sun,Li; Chen,Yanxia; Zhang,Lei; Zhang,Zhenghui

doi:10.3892/etm.2017.5092

Therapeutic effects of stemonine on particulate matter 2.5‑induced chronic obstructive pulmonary disease in mice

Authors:
- Jinbo Zhang
- Shiqing Li
- Li Sun
- Yanxia Chen
- Lei Zhang
- Zhenghui Zhang
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Affiliations: Center for Preventive Treatment of Disease, Yantai Hospital of Traditional Chinese Medicine, Yantai, Shandong 264016, P.R. China, Department of Encephalopathy, Yantai Hospital of Traditional Chinese Medicine, Yantai, Shandong 264016, P.R. China, Department of Gynecology and Obstetrics, Yantai Hospital of Traditional Chinese Medicine, Yantai, Shandong 264016, P.R. China, Department of Rehabilitation, Yantai Hospital of Traditional Chinese Medicine, Yantai, Shandong 264016, P.R. China, Department of Heart Disease, Yantai Hospital of Traditional Chinese Medicine, Yantai, Shandong 264016, P.R. China
Published online on: September 1, 2017 https://doi.org/10.3892/etm.2017.5092
Pages: 4453-4459

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Abstract

Particulate matter 2.5 (PM2.5) is a growing concern worldwide due to its association with respiratory diseases, including chronic obstructive pulmonary disease (COPD). Stemonine, a traditional Chinese herb, has been demonstrated to exhibit anti‑inflammatory and antioxidant properties, making it a potential drug for the treatment of respiratory diseases. The therapeutic effects of stemonine on mice with PM2.5‑induced COPD were investigated in the present study. Kunming mice were randomly divided into the following five groups (n=10/group): Control, model, low‑dose stemonine, moderate‑dose stemonine and high‑dose stemonine. The model mice received an intranasal instillation of PM2.5 suspension (40 mg/kg). The levels of specific enzymes, markers of oxidative stress, and the inflammatory cytokines tumor necrosis factor (TNF)‑α and interleukin (IL)‑6 were measured in the bronchoalveolar lavage fluid of the mice using ELISA kits. Hematoxylin and eosin staining was performed to determine inflammatory changes to the lung tissue. It was demonstrated that stemonine could significantly alleviate lung injury by decreasing the levels of enzymes and cytokines associated with inflammation and oxidative stress in a dose‑dependent manner. In addition, stemonine dose‑dependently increased the amount of superoxide dismutase. These results suggest that stemonine reduces lung inflammation in mice with PM2.5‑induced COPD, providing a novel approach for the treatment of PM2.5‑induced respiratory diseases.

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1	Kaiser J: Air pollution: Evidence mounts that tiny particles can kill. Science. 289:22–23. 2000. View Article : Google Scholar : PubMed/NCBI
2	Zhao Q, He KB, Ma YL, Jia YT, Cheng Y, Liu H and Wang SW: Regional PM pollution in Beijing and surrounding area during summertime. Huan Jing Ke Xue. 30:1873–1880. 2009.(In Chinese). PubMed/NCBI
3	Pope CA III, Burnett RT, Thun MJ, Calle EE, Krewski D, Ito K and Thurston GD: Lung cancer, cardiopulmonary mortality and long-term exposure to fine particulate air pollution. JAMA. 287:1132–1141. 2002. View Article : Google Scholar : PubMed/NCBI
4	Kodavanti UP, Jaskot RH, Su WY, Costa DL, Ghio AJ and Dreher KL: Genetic variability in combustion particle-induced chronic lung injury. Am J Physiol. 272:L521–L532. 1997.PubMed/NCBI
5	Tong Y, Ni X, Zhang Y, Chen F, Zhang G and Ye S: Study of toxicological mechanism of acidified aerosols. Biol Trace Elem Res. 85:149–156. 2002. View Article : Google Scholar : PubMed/NCBI
6	Ogino K, Zhang R, Takahashi H, Takemoto K, Kubo M, Murakami I, Wang DH and Fujikura Y: Allergic airway inflammation by nasal inoculation of particulate matter (PM2.5) in NC/Nga mice. PloS One. 9:e927102014. View Article : Google Scholar : PubMed/NCBI
7	Dye JA, Lehmann JR, McGee JK, Winsett DW, Ledbetter AD, Everitt JI, Ghio AJ and Costa DL: Acute pulmonary toxicity of particulate matter filter extracts in rats: Conherence with epidemiologic studies in Utah valley residents. Environ Health Perspect. 109 Suppl 3:S395–S403. 2001. View Article : Google Scholar
8	Greenwell LL, Moreno T, Jones TP and Richards RJ: Particle-induced oxidative damage is ameliorated by pulmonary antioxidants. Free Radic Biol Med. 32:898–905. 2002. View Article : Google Scholar : PubMed/NCBI
9	Cazzola M, Donner CF and Hanania NA: One hundred years of chronic obstructive pulmonary disease (COPD). Respir Med. 101:1049–1065. 2007. View Article : Google Scholar : PubMed/NCBI
10	Bozarth AL, Covey A, Gohar A and Salzman G: Chronic obstructive pulmonary disease: Clinical review and update on consensus guidelines. Hosp Pract. 42:79–91. 2014. View Article : Google Scholar
11	Kammerl IE, Dann A, Mossina A, Brech D, Lukas C, Vosyka O, Nathan P, Conlon TM, Wagner DE, Overkleeft HS, et al: Impairment of immunoproteasome function by cigarette smoke and in chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 193:1230–1241. 2016. View Article : Google Scholar : PubMed/NCBI
12	Stefan MS, Rothberg MB, Shieh MS, Pekow PS and Lindenauer PK: Association between antibiotic treatment and outcomes in patients hospitalized with acute exacerbation of COPD treated with systemic steroids. Chest. 143:82–90. 2013. View Article : Google Scholar : PubMed/NCBI
13	Chen J, Zhou H, Wang J, Zhang B, Liu F, Huang J, Li J, Lin J, Bai J and Liu R: Therapeutic effects of resveratrol in a mouse model of HDM-induced allergic asthma. Int Immunopharmacol. 25:43–48. 2015. View Article : Google Scholar : PubMed/NCBI
14	Xiao XQ, Lai J, Luo YL, Wang HB, Fang YL and Huang SF: Study on reversal of effect of double coptis in vivo on the resistance of Klebsiella pneumoniae. J Gannan Med Univ. 36:190–192. 2016.(In Chinese).
15	Zhang F: Clinical observation on treating ventilator-associated pneumonia with the Qingjin Huatan decoction. Clin J Chin Med. 8:71–72. 2016.(In Chinese).
16	Wright J and Paauw DS: Complications of antibiotic therapy. Med Clin North Am. 97:667–679. 2013. View Article : Google Scholar : PubMed/NCBI
17	Blumenthal KG, Shenoy ES, Hurwitz S, Varughese CA, Hooper DC and Banerji A: Effect of a drug allergy educational program and antibiotic prescribing guideline on inpatient clinical providers' antibiotic prescribing knowledge. J Allergy Clin Immunol Pract. 2:407–413. 2014. View Article : Google Scholar : PubMed/NCBI
18	Rolain JM, Abat C, Jimeno MT, Fournier PE and Raoult D: Do we need new antibiotics? Clin Microbiol Infect. 22:408–415. 2016. View Article : Google Scholar : PubMed/NCBI
19	Xu YT, Shaw PC, Jiang RW, Hon PM, Chan YM and But PP: Antitussive and central respiratory depressant effects of Stemona tuberosa. J Ethnopharmacol. 128:679–684. 2010. View Article : Google Scholar : PubMed/NCBI
20	Gao JF and Zhang XY: Experimental observation on germicidal efficacy of a herbal compound disinfectant solution. Chin J Disinfection. 22:305–306. 2005.
21	Chung HS, Hon PM, Lin G, But PP and Dong H: Antitussive activity of Stemona alkaloids from Stemona tuberose. Planta Med. 69:914–920. 2003. View Article : Google Scholar : PubMed/NCBI
22	Liao JF, Shi CC, Chen SY, Fu YT and Chen CF: Spasmolytic effect of walter extract of Stemonae radix on the guinea-pig tracheal smooth muscle in vitro. J Ethnopharmacol. 57:57–62. 1997. View Article : Google Scholar : PubMed/NCBI
23	Wang X, Hai CX, Liang X, Yu SX, Zhang W and Li YL: The protective effects of acanthopanax senticosus, harms aqueous extracts against oxidative stress: Role of Nrf2 and antioxidant enzymes. J Ethnopharmacol. 127:424–432. 2010. View Article : Google Scholar : PubMed/NCBI
24	Onizawa S, Aoshiba K, Kajita M, Miyamoto Y and Nagai A: Platinum nanoparticle antioxidants inhibit pulmonary inflammation in mice exposed to cigarette smoke. Pulm Pharmacol Ther. 22:340–349. 2009. View Article : Google Scholar : PubMed/NCBI
25	Rahman I, Bismas SK and Kode A: Oxidant and antioxidant balance in the airways and airway disease. Eur J Pharmacol. 533:222–239. 2006. View Article : Google Scholar : PubMed/NCBI
26	Driscoll KE: TNFα and MIP-2: Role in particle-induced inflammation and regulation by oxidative stress. Toxicol Lett. 112–113:177–183. 2000. View Article : Google Scholar
27	Cui S, He ZZ, Zhu ZW, Sun Z, Xu YT, Wang JL, Bao YY, Ji DY, Liu S, Liu JT, et al: Microfluidic analysis of PM2.5-induced epithelial-mesenchymal transition in human bronchial epithelial 16HBE cells. Microfluid Nanofluidics. 19:263–272. 2015. View Article : Google Scholar
28	Jing Y, Zhang H, Cai Z, Zhao Y, Wu Y, Zheng X, Liu Y, Qin Y, Gu M and Jin J: Bufei huoxue capsule attenuates PM2.5-induced pulmonary inflammation in mice. Evid Based Complement Alternat Med. 2017:15757932017. View Article : Google Scholar : PubMed/NCBI
29	Wang H, Song L, Ju W, Wang X, Dong L, Zhang Y, Ya P, Yang C and Li F: The acute airway inflammation induced by PM2.5 exposure and the treatment of essential oils in Balb/c mice. Sci Rep. 7:442562017. View Article : Google Scholar : PubMed/NCBI
30	Jung KH, Beak H, Park S, Shin D, Jung J, Park S, Kim J and Bae H: The therapeutic effects of tuberostemonine against cigarette smoke-induced acute lung inflammation in mice. Eur J Pharmacol. 774:80–86. 2016. View Article : Google Scholar : PubMed/NCBI
31	Qin YY, Jing Y, Liu Y, Gu MJ, Jin J, Pan L, Cai Z and Zhang HC: Influence of Guben Zhike Granules on lung function and morphology of lung injury mouse model induced by PM_2.5. China J Trad Chin Med Pharm. 31:1028–L1031. 2016.(In Chinese).
32	Park SH, Chen WC, Durmus N, Bleck B, Reibman J, Riemekasten G and Grunig G: The effects of antigen-specific IgG1 antibody for the pulmonary-hypertension-phenotype and B cells for inflammation in mice exposed to antigen and fine particles from air pollution. PLoS One. 10:e01299102015. View Article : Google Scholar : PubMed/NCBI
33	Su WY, Jaskot RH, Richards J, Abramson SR, Woessner JF Jr, Yu WH and Dreher KL: Induction of pulmonary matrilysin expression by combustion and ambient air particles. Am J Physiol Lung Cell Mol Physiol. 279:L152–L160. 2000.PubMed/NCBI
34	Veranth JM, Kaser EG, Veranth MM, Koch M and Yost GS: Cytokine responses of human lung cells (BEAS-2B) treated with micron-sized and nanoparticles of metal oxides compared to soil dusts. Part Fibre Toxicol. 4:22007. View Article : Google Scholar : PubMed/NCBI