Bacterial keratitis: Photodynamic inactivation reduced experimental inflammation

Deichelbohrer,Mona; Wu,Ming‑Feng; Seitz,Berthold; Hüttenberger,Dirk; Laschke,Matthias W.; Foth,Hans‑Jochen; Wonnenberg,Bodo; Wagenpfeil,Stefan; Meier,Carola; Bischoff,Markus; Tschernig,Thomas

doi:10.3892/etm.2017.5109

Bacterial keratitis: Photodynamic inactivation reduced experimental inflammation

Authors:
- Mona Deichelbohrer
- Ming‑Feng Wu
- Berthold Seitz
- Dirk Hüttenberger
- Matthias W. Laschke
- Hans‑Jochen Foth
- Bodo Wonnenberg
- Stefan Wagenpfeil
- Carola Meier
- Markus Bischoff
- Thomas Tschernig
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Affiliations: Institute of Anatomy and Cell Biology, Medical Faculty, Saarland University, D‑66424 Homburg/Saar, Germany, Department of Ophthalmology, Saarland University Medical Center/UKS, D‑66424 Homburg/Saar, Germany, APO‑CARE Pharma GmbH, D‑33647 Bielefeld, Germany, Institute of Clinical and Experimental Surgery, Saarland University, Medical Faculty, D‑66424 Homburg/Saar, Germany, Department of Physics, University of Kaiserslautern, D‑67663 Kaiserslautern, Germany, Institute for Medical Biometry, Epidemiology and Medical Informatics, Saarland University, Medical Faculty, D‑66424 Homburg/Saar, Germany, Institute of Medical Microbiology and Hygiene, Saarland University, Medical Faculty, D‑66424 Homburg/Saar, Germany
Published online on: September 5, 2017 https://doi.org/10.3892/etm.2017.5109
Pages: 4509-4514

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Abstract

The successful treatment of bacterial keratitis remains an unsolved clinical problem. The current study aimed to establish a murine keratitis model and to investigate the effect of chlorin e6 (Ce6) and photodynamic inactivation (PDI) on corneal inflammation. The cornea of anesthetized mice was scratched and covered with a bacterial suspension of Pseudomonas aeruginosa. A paste containing Ce6 was applied to the cornea with subsequent exposure to specified light. Two days later the animals were sacrificed, and the globes were processed for light microscopy. Evaluation parameters were the maximal corneal thickness and the severity of the hypopyon. The maximal corneal thickness of 290±16 µm in the infected and untreated group was significantly reduced to 220±8 µm in the infected and treated group (P<0.05). In addition, the hypopyon was less severe in the infected and treated group. In conclusion, the present study indicates that PDI using Ce6 may be a potential approach to treat patients suffering with severe bacterial keratitis.

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1	Ong HS and Corbett MC: Corneal infections in the 21st century. Postgrad Med J. 91:565–571. 2015. View Article : Google Scholar : PubMed/NCBI
2	Taube MA, del Mar Cendra M, Elsahn A, Christodoulides M and Hossain P: Pattern recognition receptors in microbial keratitis. Eye (Lond). 29:1399–1415. 2015. View Article : Google Scholar : PubMed/NCBI
3	Young G, Young AG and Lakkis C: Review of complications associated with contact lenses from unregulated sources of supply. Eye Contact Lens. 40:58–64. 2014. View Article : Google Scholar : PubMed/NCBI
4	Lorenzo-Morales J, Khan NA and Walochnik J: An update on Acanthamoeba keratitis: Diagnosis, pathogenesis and treatment. Parasite. 22:102015. View Article : Google Scholar : PubMed/NCBI
5	Makdoumi K, Mortensen J, Sorkhabi O, Malmvall BE and Crafoord S: UVA-riboflavin photochemical therapy of bacterial keratitis: A pilot study. Graefes Arch Clin Exp Ophthalmol. 250:95–102. 2012. View Article : Google Scholar : PubMed/NCBI
6	Stachon T, Wang J, Eppig T, Langenbucher A, Bischoff M, Seitz B and Szentmáry N: KGF, FGFb, VEGF, HGF and TGFβ1 secretion of human keratocytes following photodynamic inactivation (PDI) in vitro. Graefes Arch Clin Exp Ophthalmol. 251:1987–1993. 2013. View Article : Google Scholar : PubMed/NCBI
7	Stachon T, Wang J, Song X, Langenbucher A, Seitz B and Szentmáry N: Impact of crosslinking/riboflavin-UVA-photodynamic inactivation on viability, apoptosis and activation of human keratocytes in vitro. J Biomed Res. 29:321–325. 2015.PubMed/NCBI
8	Wu MF, Stachon T, Wang J, Song X, Colanesi S, Seitz B, Wagenpfeil S, Langenbucher A and Szentmáry N: Effect of keratocyte supernatant on epithelial cell migration and proliferation after corneal crosslinking (CXL). Curr Eye Res. 41:466–473. 2016. View Article : Google Scholar : PubMed/NCBI
9	Randleman JB, Khandelwal SS and Hafezi F: Corneal cross-linking. Surv Ophthalmol. 60:509–523. 2015. View Article : Google Scholar : PubMed/NCBI
10	Papaioannou L, Miligkos M and Papathanassiou M: Corneal collagen cross-linking for infectious keratitis: A systematic review and meta-analysis. Cornea. 35:62–71. 2016. View Article : Google Scholar : PubMed/NCBI
11	Wang J, Stachon T, Eppig T, Langenbucher A, Seitz B and Szentmáry N: Impact of photodynamic inactivation (PDI) using the photosensitizer chlorin e6 on viability, apoptosis and proliferation of human keratocytes in vitro. Graefes Arch Clin Exp Ophthalmol. 251:2725–2731. 2013. View Article : Google Scholar : PubMed/NCBI
12	Wang J, Stachon T, Eppig T, Langenbucher A, Seitz B and Szentmáry N: Impact of photodynamic inactivation (PDI) using the photosensitizer chlorin e6 on viability, apoptosis, and proliferation of human corneal endothelial cells. Graefes Arch Clin Exp Ophthalmol. 251:1199–1204. 2013. View Article : Google Scholar : PubMed/NCBI
13	Rovaldi CR, Pievsky A, Sole NA, Friden PM, Rothstein DM and Spacciapoli P: Photoactive porphyrin derivative with broad-spectrum activity against oral pathogens In vitro. Antimicrob Agents Chemother. 44:3364–3367. 2000. View Article : Google Scholar : PubMed/NCBI
14	Gad F, Zahra T, Francis KP, Hasan T and Hamblin MR: Targeted photodynamic therapy of established soft-tissue infections in mice. Photochem Photobiol Sci. 3:451–458. 2004. View Article : Google Scholar : PubMed/NCBI
15	Park JH, Moon YH, Bang IS, Kim YC, Kim SA, Ahn SG and Yoon JH: Antimicrobial effect of photodynamic therapy using a highly pure chlorin e6. Lasers Med Sci. 25:705–710. 2010. View Article : Google Scholar : PubMed/NCBI
16	Martins SA, Combs JC, Noguera G, Camacho W, Wittmann P, Walther R, Cano M, Dick J and Behrens A: Antimicrobial efficacy of riboflavin/UVA combination in vitro for bacterial and fungal isolates: A potential new treatment for infectious keratitis. Invest Ophthalmol Vis Sci. 49:3402–3408. 2008. View Article : Google Scholar : PubMed/NCBI
17	Khan YA, Kashiwabuchi RT, Martins SA, Castro-Combs JM, Kalyani S, Stanley P, Flikier D and Behrens A: Riboflavin and ultraviolet light a therapy as an adjuvant treatment for medically refractive Acanthamoeba keratitis: Report of 3 cases. Ophthalmology. 118:324–331. 2011. View Article : Google Scholar : PubMed/NCBI
18	Ekanayaka SA, McClellan SA, Barrett RP, Kharotia S and Hazlett LD: Glycyrrhizin reduces HMGB1 and bacterial load in Pseudomonas aeruginosa keratitis. Invest Ophthalmol Vis Sci. 57:5799–5809. 2016. View Article : Google Scholar : PubMed/NCBI
19	Girgis DO, Sloop GD, Reed JM and O'Callaghan RJ: Susceptibility of aged mice to Staphylococcus aureus keratitis. Curr Eye Res. 29:269–275. 2004. View Article : Google Scholar : PubMed/NCBI
20	National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory AnimalsGuide for the Care and Use of Laboratory Animals. 8th. National Academies Press (US); Washington (DC): 2011, PubMed/NCBI
21	Pinto JG, Pereira AH, de Oliveira MA, Kurachi C, Raniero LJ and Ferreira-Strixino J: Chlorin E6 phototoxicity in L. major and L. braziliensis promastigotes-in vitro study. Photodiagnosis Photodyn Ther. 15:19–24. 2016.(In Press). View Article : Google Scholar : PubMed/NCBI
22	Marquart ME: Animal models of bacterial keratitis. J Biomed Biotechnol. 2011:6806422011. View Article : Google Scholar : PubMed/NCBI