SVP‑B5 peptide from Buthus martensii Karsch scorpion venom exerts hyperproliferative effects on irradiated hematopoietic cells

Wang,Yan; Xing,Baiqian; Li,Ting; Wang,Caixia; Zhou,Meixun; Liu,Yamin; Fan,Lingjie; Hu,Lili; Peng,Xiang; Xiang,Yongxin; Wang,Han; Kong,Tianhan; Dong,Weihua; Guo,Qifeng

doi:10.3892/etm.2017.5152

SVP‑B5 peptide from Buthus martensii Karsch scorpion venom exerts hyperproliferative effects on irradiated hematopoietic cells

Authors:
- Yan Wang
- Baiqian Xing
- Ting Li
- Caixia Wang
- Meixun Zhou
- Yamin Liu
- Lingjie Fan
- Lili Hu
- Xiang Peng
- Yongxin Xiang
- Han Wang
- Tianhan Kong
- Weihua Dong
- Qifeng Guo
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Affiliations: Department of Orthopedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China, Department of Pathophysiology, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China, Department of Hematology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China
Published online on: September 20, 2017 https://doi.org/10.3892/etm.2017.5152
Pages: 5081-5086

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Abstract

Previous studies have demonstrated the radioprotective efficacy of scorpion venom peptide, fraction II (SVPII) from the venom of Buthus martensii Karsch. In the present study, the SVP‑B5 polypeptide, which is one of the active components of SVPII, was purified using a two‑step chromatographic process. SVP‑B5 significantly promoted the proliferation of irradiated M‑NFS‑60 mouse‑derived myelocytic leukemia cells. In addition, SVP‑B5 effectively and persistently promoted hematopoietic recovery and expansion of hematopoietic cells after irradiation as demonstrated by cobblestone area forming cell and long‑term bone marrow culture assays. Treatment of M‑NFS‑60 cells with SVP‑B5 upregulated the expression of interleukin 3 receptor and activated the Janus kinase‑2/signal transducer and activator of transcription 5 signaling pathway. In conclusion, the present study demonstrated that SVP‑B5 has growth factor‑like properties and may be used as a therapeutic modality in the recovery of severe myelosuppression, which is a common side effect of radiotherapy.

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