BMP‑7 accelerates the differentiation of rabbit mesenchymal stem cells into cartilage through the Wnt/β‑catenin pathway

Fu,Hong‑Dan; Wang,Hai‑Rui; Li,Dai‑He

doi:10.3892/etm.2017.5210

BMP‑7 accelerates the differentiation of rabbit mesenchymal stem cells into cartilage through the Wnt/β‑catenin pathway

Authors:
- Hong‑Dan Fu
- Hai‑Rui Wang
- Dai‑He Li
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Affiliations: Immunization Section, Inner Mongolia Medical University, Hohhot, Inner Mongolia 010010, P.R. China, Department of Orthopedics, The Affiliated People's Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010010, P.R. China, Department of Orthopedics, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010030, P.R. China
Published online on: September 27, 2017 https://doi.org/10.3892/etm.2017.5210
Pages: 5424-5428
Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mesenchymal stem cells (MSCs) are able to differentiate into adipocytes, chondroblasts or cartilage under different stimulation conditions. Identifying a mechanism that triggers the differentiation of MSCs into cartilage may help the development of novel therapeutic approaches for heterotopic ossification, the pathological formation of lamellar bone in soft tissue outside the skeleton that may lead to debilitating immobility. Bone morphogenetic proteins (BMPs), including BMP‑7, are the most potent growth factors for enhancing bone formation. The current study aimed to understand the potential involvement of the Wnt/β‑catenin signaling pathway in the BMP‑7‑induced growth of rabbit MSCs (rMSCs). Different concentrations of BMP‑7 were applied to cultured rMSCs, and proliferation was evaluated by MTT assay. Changes in the phosphorylation state of glycogen synthase kinase (GSK)‑3β, in addition to the expression levels of alkaline phosphatase, β‑catenin and runt‑related transcription factor 2 were observed by western blot analysis. Following treatment with BMP‑7, the phosphorylation of GSK‑3β was stimulated and the expression of β‑catenin, ALP and Runx2 was increased. Furthermore, inhibiting β‑catenin signaling with XAV‑939 suppressed the BMP‑7‑mediated changes. The results indicated that the BMP‑7‑induced differentiation of rMSCs into cartilage was promoted primarily by the Wnt/β‑catenin pathway.

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