Isoforskolin downregulates proinflammatory responses induced by Borrelia burgdorferi basic membrane protein A

Zhao,Hua; Liu,Aihua; Shen,Longqiang; Xu,Cuiping; Zhu,Ziwei; Yang,Jiaru; Han,Xinling; Bao,Fukai; Yang,Weimin

doi:10.3892/etm.2017.5300

Isoforskolin downregulates proinflammatory responses induced by Borrelia burgdorferi basic membrane protein A

Authors:
- Hua Zhao
- Aihua Liu
- Longqiang Shen
- Cuiping Xu
- Ziwei Zhu
- Jiaru Yang
- Xinling Han
- Fukai Bao
- Weimin Yang
View Affiliations

Affiliations: Department of Microbiology and Immunology, Kunming Medical University, Kunming, Yunnan 650500, P.R. China, Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan 650500, P.R. China, School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, Yunnan 650500, P.R. China
Published online on: October 13, 2017 https://doi.org/10.3892/etm.2017.5300
Pages: 5974-5980
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The plant Coleus forskohlii is distributed primarily in India, Thailand, China, Egypt and Brazil and has a history of use in the treatment of multiple diseases. Isoforskolin (ISOF) is the principle active component of C. forskohlii native to China and has previously been studied for its biological effects. The aim of the present study was to evaluate the effect of ISOF on the proinflammatory responses induced by recombinant Borrelia burgdorferi basic membrane protein A (rBmpA). In in vitro experiments, the proinflammatory effects of rBmpA and the anti‑inflammatory function of ISOF were evaluated in murine macrophages, human macrophages and dendritic cells by detecting the transcription and expression of tumor necrosis factor (TNF)‑α and interleukin (IL)‑6. In in vivo experiments, mean arthritis index and X‑ray and histopathological examinations were used to verify the role of ISOF in experimental Lyme arthritis in mice. The results indicated that rBmpA, which induced the transcription and expression of TNF‑α and IL‑6, activated proinflammatory responses in murine macrophages, human macrophages and dendritic cells. In turn, ISOF downregulated the transcription and expression of TNF‑α and IL‑6 induced by rBmpA. Additionally, the in vivo experiments demonstrated that ISOF could also inhibit the symptoms of experimental Lyme arthritis. These results suggest that ISOF may have a potential application as an anti‑inflammatory agent for the treatment of Lyme arthritis.

View Figures

View References

1	Gerstenblith TA and Stern TA: Lyme disease: A review of its epidemiology, evaluation, and treatment. Psychosomatics. 55:421–429. 2014. View Article : Google Scholar : PubMed/NCBI
2	Stanek G, Wormser GP, Gray J and Strle F: Lyme borreliosis. Lancet. 379:461–473. 2012. View Article : Google Scholar : PubMed/NCBI
3	Oosting M, Buffen K, van der Meer JW, Netea MG and Joosten LA: Innate immunity networks during infection with Borrelia burgdorferi. Crit Rev Microbiol. 42:233–244. 2016.PubMed/NCBI
4	Pal U, Wang P, Bao F, Yang X, Samanta S, Schoen R, Wormser GP, Schwartz I and Fikrig E: Borrelia burgdorferi basic membrane proteins A and B participate in the genesis of Lyme arthritis. J Exp Med. 205:133–141. 2008. View Article : Google Scholar : PubMed/NCBI
5	Yang X, Izadi H, Coleman AS, Wang P, Ma Y, Fikrig E, Anguita J and Pal U: Borrelia burgdorferi lipoprotein BmpA activates proinflammatory responses in human synovial cells through a protein way. Microbes Infect. 10:1300–1308. 2008. View Article : Google Scholar : PubMed/NCBI
6	Yin J, Wang Y, Tan B, Kang Y, Xie D, Tian L and Huang J: Matrix solid-phase dispersion extraction for chromatographic analysis of labdane diterpenoids in Coleus forskohlii. Phytochem Anal. 24:117–123. 2013. View Article : Google Scholar : PubMed/NCBI
7	Tian L, Wang Y, Ling Y, Yin J, Chen J and Huang J: A sensitive and specific HPLC-MS/MS analysis and preliminary pharmacokinetic characterization of isoforskolin in beagle dogs. J Chromatogr B Analyt Technol Biomed Life Sci. 879:3688–3693. 2011. View Article : Google Scholar : PubMed/NCBI
8	Feng S, Wang LP, Voravuthikunchai S, Liang Z, Liu AH and Bao FK: Isoforskolin from native plant Coleus forskohlii of Yunnan, China plays multiple biological roles. Open J Immunol. 6:63–66. 2016. View Article : Google Scholar
9	Yang WM, Qiang DQ, Zhang M, Ma L, Zhang Y, Qing C, Xu Y, Zhen C, Liu J and Chen YH: Isoforskolin pretreatment attenuates lipopolysaccharide-induced acute lung injury in animal models. Int Immunopharmacol. 11:683–692. 2011. View Article : Google Scholar : PubMed/NCBI
10	Ringseis R, Schulz N, Saal D and Eder K: Troglitazone but not conjugated linoleic acid reduces gene expression and activity of matrix-metalloproteinases-2 and −9 in PMA-differentiated THP-1 macrophages. J Nutr Biochem. 19:594–603. 2008. View Article : Google Scholar : PubMed/NCBI
11	Berges C, Naujokat C, Tinapp S, Wieczorek H, Höh A, Sadeghi M, Opelz G and Daniel V: A cell line model for the differentiation of human dendritic cells. Biochem Bioph Res Comm. 333:896–907. 2005. View Article : Google Scholar
12	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
13	U.S. Department of Health And Human Services, National Institutes of Health, Office of Laboratory Animal Welfare: Public Health Service Policy on Humane Care and Use of Laboratory Animals, Revised 2015, NIH Publication No. 15–8013Office of Laboratory Animal Welfare, National Institutes of Health. U.S. Department of Health and Human Services; Bethesda, MD, USA:
14	Nowalk AJ, Gilmore RD Jr and Carroll JA: Serologic proteome analysis of Borrelia burgdorferi membrane-associated proteins. Infect Immun. 74:3864–3873. 2006. View Article : Google Scholar : PubMed/NCBI
15	Bryksin AV, Tomova A, Godfrey HP and Cabello FC: BmpA is a surface-exposed outer membrane protein of Borrelia burgdorferi. FEMS Microbiol Lett. 309:77–83. 2010.PubMed/NCBI
16	Hu L: Lyme Arthritis. Infect Dis Clin N Am. 19:947–961. 2005. View Article : Google Scholar
17	Cervantes JL, Hawley KL, Benjamin SJ, Weinerman B, Luu SM and Salazar JC: Phagosomal TLR signaling upon Borrelia burgdorferi infection. Front Cell Infect Microbiol. 4:552014.PubMed/NCBI
18	Zanoni I and Granucci F: Differences in lipopolysaccharide-induced signaling between conventional dendritic cells and macrophages. Immunobiology. 215:709–712. 2010. View Article : Google Scholar : PubMed/NCBI
19	Chow JC, Young DW, Golenbock DT, Christ WJ and Gusovsky F: Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction. J Biol Chem. 274:10689–10692. 1999. View Article : Google Scholar : PubMed/NCBI