Jinwei Tang modulates HDAC2 expression in a rat model of COPD

  • Authors:
    • Jianjun Wu
    • Xin Li
    • Yang Qin
    • Juan Cheng
    • Gaimei Hao
    • Ruifeng Jin
    • Chenjun Zhu
  • View Affiliations

  • Published online on: January 5, 2018     https://doi.org/10.3892/etm.2018.5707
  • Pages: 2604-2610
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Abstract

The aim of the present study was to investigate the effect of a Traditional Chinese Herbal Medicine (TCHM), named Jinwei Tang on histone deacetylase 2 (HDAC2) and its role in the regulation of corticosteroid resistance in a rat model of chronic obstructive pulmonary disease (COPD). Male Wistar rats were divided into five groups (each n=10): COPD group, established by the intratracheal instillation of lipopolysaccharide and passive smoke exposure, and control, budesonide, theophylline + budesonide and Jinwei Tang + budesonide groups. Lung function was measured, lung tissue histopathology was examined and HDAC2 expression in the lung was assessed by immunohistochemistry. In addition, protein levels of interleukin‑8 (IL‑8), tumor necrosis factor (TNF)‑α and HDAC2 in lung homogenate were quantified by ELISA. The rat COPD model exhibited alterations of the ratio of forced expiratory volume in 0.2 sec (FEV0.2) to the forced vital capacity, FEV0.2, dynamic compliance and airway resistance. HDAC2 expression was markedly reduced in the lung tissue of the COPD group compared with the control group, and treatment with Jinwei Tang + budesonide or theophylline + budesonide resulted in significant attenuation of the reduction of HDAC2 expression in the lungs (P<0.05). However, treatment with budesonide alone did not significantly alter HDAC2 expression. In the Jinwei Tang + budesonide and theophylline + budesonide groups, IL‑8 and TNF‑α expression was significantly decreased (P<0.05) and the HDAC2 level increased (P<0.05) compared with that in the COPD group. In conclusion, Jinwei Tang modulates airway inflammation and may enhance the anti‑inflammatory effect of glucocorticoid through the upregulation of HADC2 expression in a rat model of COPD.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wu J, Li X, Qin Y, Cheng J, Hao G, Jin R and Zhu C: Jinwei Tang modulates HDAC2 expression in a rat model of COPD. Exp Ther Med 15: 2604-2610, 2018
APA
Wu, J., Li, X., Qin, Y., Cheng, J., Hao, G., Jin, R., & Zhu, C. (2018). Jinwei Tang modulates HDAC2 expression in a rat model of COPD. Experimental and Therapeutic Medicine, 15, 2604-2610. https://doi.org/10.3892/etm.2018.5707
MLA
Wu, J., Li, X., Qin, Y., Cheng, J., Hao, G., Jin, R., Zhu, C."Jinwei Tang modulates HDAC2 expression in a rat model of COPD". Experimental and Therapeutic Medicine 15.3 (2018): 2604-2610.
Chicago
Wu, J., Li, X., Qin, Y., Cheng, J., Hao, G., Jin, R., Zhu, C."Jinwei Tang modulates HDAC2 expression in a rat model of COPD". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2604-2610. https://doi.org/10.3892/etm.2018.5707