Open Access

Protective effect of black garlic extracts on tert-Butyl hydroperoxide-induced injury in hepatocytes via a c-Jun N-terminal kinase-dependent mechanism

  • Authors:
    • Ko‑Chao Lee
    • Chih‑Chuan Teng
    • Chien‑Heng Shen
    • Wen‑Shih Huang
    • Chien‑Chang Lu
    • Hsing‑Chun Kuo
    • Shui‑Yi Tung
  • View Affiliations

  • Published online on: January 8, 2018     https://doi.org/10.3892/etm.2018.5719
  • Pages: 2468-2474
  • Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Black garlic has been reported to show multiple bioactivities against the development of different diseases. In the present study, the hepatoprotective effect of black garlic on injured liver cells was investigated. Rat clone‑9 hepatocytes were used for all experiments; tert‑Butyl hydroperoxide (tBHP) was used to induce injury of rat clone‑9 hepatocytes. The contents of malondialdehyde (MDA) and glutathione (GSH); anti‑oxidative enzyme activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx); and mRNA expression levels of interleukin (IL)‑6 and IL‑8 in rat clone‑9 hepatocytes were determined to evaluate the level of cell damage. Black garlic extracts were demonstrated to significantly attenuate tBHP‑induced cell death of rat clone‑9 hepatocytes (P<0.05). Pretreatment with black garlic extracts antagonized GSH depletion, tBHP‑increased MDA accumulation and the mRNA expression level of IL‑6/IL‑8, and tBHP‑decreased antioxidative enzyme activities (all P<0.05). Moreover, the present study revealed that c‑Jun N‑terminal kinase signaling regulated black garlic‑inhibited tBHP effects in rat clone‑9 hepatocytes. Our findings demonstrate that black garlic has the hepatoprotective potential to block tBHP‑damaged effects on cell death, lipid peroxidation, oxidative stress, and inflammation in rat clone‑9 hepatocytes. Thus, the present study indicates that black garlic may be an excellent natural candidate in the development of adjuvant therapy and healthy foods for liver protection.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Lee KC, Teng CC, Shen CH, Huang WS, Lu CC, Kuo HC and Tung SY: Protective effect of black garlic extracts on tert-Butyl hydroperoxide-induced injury in hepatocytes via a c-Jun N-terminal kinase-dependent mechanism. Exp Ther Med 15: 2468-2474, 2018
APA
Lee, K., Teng, C., Shen, C., Huang, W., Lu, C., Kuo, H., & Tung, S. (2018). Protective effect of black garlic extracts on tert-Butyl hydroperoxide-induced injury in hepatocytes via a c-Jun N-terminal kinase-dependent mechanism. Experimental and Therapeutic Medicine, 15, 2468-2474. https://doi.org/10.3892/etm.2018.5719
MLA
Lee, K., Teng, C., Shen, C., Huang, W., Lu, C., Kuo, H., Tung, S."Protective effect of black garlic extracts on tert-Butyl hydroperoxide-induced injury in hepatocytes via a c-Jun N-terminal kinase-dependent mechanism". Experimental and Therapeutic Medicine 15.3 (2018): 2468-2474.
Chicago
Lee, K., Teng, C., Shen, C., Huang, W., Lu, C., Kuo, H., Tung, S."Protective effect of black garlic extracts on tert-Butyl hydroperoxide-induced injury in hepatocytes via a c-Jun N-terminal kinase-dependent mechanism". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2468-2474. https://doi.org/10.3892/etm.2018.5719