Open Access

Establishment of different experimental asthma models in mice

  • Authors:
    • Qian‑Lin Yu
    • Zhangbo Chen
  • View Affiliations

  • Published online on: January 8, 2018     https://doi.org/10.3892/etm.2018.5721
  • Pages: 2492-2498
  • Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Asthma is a complex disease. The heterogeneity of airway inflammation during asthma indicates there are different mechanisms involved. In order to further study the mechanisms of asthma, different mouse models were established to mimic corresponding subtypes of asthma in clinic. Eosinophilic asthma was established by intraperitoneal injections of ovalbumin (OVA) on day 0 and day 7, followed by inhalation of aerosolized OVA on days 14‑17. Neutrophilic asthma was established by transtracheal administration of a high dose of lipopolysaccharide (LPS; 10 µg) on days 15 and 17 in combination with OVA sensitization and challenge as described previously. Mix‑granulocytic asthma was established by transtracheal administration of a low dose of LPS (1 µg) on day 15, in combination with OVA sensitization and challenge as described previously. Compared with healthy controls, increased numbers of eosinophils, elevated levels of T helper (Th)2 cytokines in bronchoalveolar lavage fluid (BALF), and moderated inflammation of lung tissues was observed in eosinophilic asthma. Increased numbers of neutrophils, elevated levels of Th1 and Th17 cytokines in BALF and severe inflammation of lung tissues was observed in neutrophilic asthma. Increased numbers of both eosinophils and neutrophils, elevated levels of Th1, Th2 and Th17 cytokines in BALF and severe inflammation of lung tissues was observed in mix-granulocytic asthma. Airway hyperresponsiveness, increased bronchial mucus secretion, and elevated serum levels of immunoglobin (Ig)E and OVA‑IgE were detected in all three asthma models. Dexamethasone reduced the pathogenic symptoms of the mice in eosinophilic asthma, however had no effect on neutrophilic asthma or mix‑granulocytic asthma. Each model of asthma established in the present study represents corresponding subtypes of asthma in clinic.
View Figures
View References

Related Articles

Journal Cover

March-2018
Volume 15 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yu QL and Chen Z: Establishment of different experimental asthma models in mice. Exp Ther Med 15: 2492-2498, 2018.
APA
Yu, Q., & Chen, Z. (2018). Establishment of different experimental asthma models in mice. Experimental and Therapeutic Medicine, 15, 2492-2498. https://doi.org/10.3892/etm.2018.5721
MLA
Yu, Q., Chen, Z."Establishment of different experimental asthma models in mice". Experimental and Therapeutic Medicine 15.3 (2018): 2492-2498.
Chicago
Yu, Q., Chen, Z."Establishment of different experimental asthma models in mice". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2492-2498. https://doi.org/10.3892/etm.2018.5721