Open Access

Biochanin A extirpates the epithelial‑mesenchymal transition in a human lung cancer

  • Authors:
    • Yan Wang
    • Jiao‑Jiao Li
    • Yu‑Min Chen
  • View Affiliations

  • Published online on: January 10, 2018     https://doi.org/10.3892/etm.2018.5731
  • Pages: 2830-2836
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The natural iso-flavonoid, biochanin A, is categorized as a phytoestrogen and has been demonstrated to exhibit various pharmacological properties. However, no effects of biochanin A on lung cancer cell lines have been reported. Therefore, the present study aimed to demonstrate whether biochanin A has the ability to reduce lung cancer triggered pro‑inflammatory effects from leukemic monocytes. We studied the release of cytokines, tumor necrosis factor (TNF)‑α and interleukin (IL)‑6, from the cocultured cells of A427:AML‑193. In addition to this, epithelial‑mesenchymal transition was also monitored. In the cocultured A427 and AML‑193, AML‑193 was stimulated by A427 cells assisting the release of TNF‑α and IL‑6 cytokines, but the addition of A427 with biochanin A reduced A427‑triggered generation of cytokines by AML‑193. Moreover, this non‑functional A427:AML‑193 coculture reduced the metastasis effects of A427 cells, as determined by wound healing assays and migration/invasion assays. These results were further confirmed by a reduction in Snail and E‑cadherin expression levels, which are indicators of the epithelial‑mesenchymal transition. These findings suggest the therapeutic effect of biochanin A against lung cancer evoked inflammation and pro‑inflammatory functions from monocytic cells.
View Figures
View References

Related Articles

Journal Cover

March-2018
Volume 15 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Li JJ and Chen YM: Biochanin A extirpates the epithelial‑mesenchymal transition in a human lung cancer. Exp Ther Med 15: 2830-2836, 2018.
APA
Wang, Y., Li, J., & Chen, Y. (2018). Biochanin A extirpates the epithelial‑mesenchymal transition in a human lung cancer. Experimental and Therapeutic Medicine, 15, 2830-2836. https://doi.org/10.3892/etm.2018.5731
MLA
Wang, Y., Li, J., Chen, Y."Biochanin A extirpates the epithelial‑mesenchymal transition in a human lung cancer". Experimental and Therapeutic Medicine 15.3 (2018): 2830-2836.
Chicago
Wang, Y., Li, J., Chen, Y."Biochanin A extirpates the epithelial‑mesenchymal transition in a human lung cancer". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2830-2836. https://doi.org/10.3892/etm.2018.5731