Open Access

Overexpression of microRNA‑506‑3p aggravates the injury of vascular endothelial cells in patients with hypertension by downregulating Beclin1 expression

  • Authors:
    • Fanfan Yi
    • Yugui Hao
    • Xiaoyi Chong
    • Wei Zhong
  • View Affiliations

  • Published online on: January 10, 2018     https://doi.org/10.3892/etm.2018.5733
  • Pages: 2844-2850
  • Copyright: © Yi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to measure the expression of microRNA (miRNA)‑506‑3p in the peripheral blood of patients with hypertension and to determine the biological functions and mechanisms of action of miR‑506‑3p. A total of 61 patients with primary hypertension were included in the present study. Peripheral blood was collected from all patients, as well as 31 healthy subjects who were included in a control group. The expression of miR‑506‑3p in peripheral blood was determined by reverse transcription‑quantitative polymerase chain reaction. Human umbilical vein endothelial cells (HUVECs) were transfected with miR‑506‑3p mimics or miR‑506‑3p inhibitor. The proliferation and migration of HUVECs were determined using cell‑counting kit 8 and Transwell assays, respectively. The cell cycle and apoptosis of HUVECs were detected by flow cytometry. The expression of Beclin1 (BECN1) protein, a potential target of miR‑506‑3p, was measured using western blotting. A dual‑luciferase reporter assay was performed to determine the interaction between BECN1 and miR‑506‑3p. It was demonstrated that miR‑506‑3p expression in the peripheral blood of patients with patients was upregulated and dependent on the severity of hypertension. miR‑506‑3p overexpression inhibited the proliferation and migration of HUVECs. In addition, miR‑506‑3p inhibited the transition from the G1 phase to the S‑phase in HUVECs. Overexpression of miR‑506‑3p promoted the apoptosis of HUVECs. Notably, miR‑506‑3p downregulated the expression of BECN1 by directly binding to its 3'‑untranslated region. The present study demonstrated that miR‑506‑3p expression is elevated in the peripheral blood of patients with hypertension and is associated with the severity of hypertension. By downregulating BECN1 expression, miR‑506‑3p aggravates injury in vascular endothelial cells by inhibiting the proliferation and migration of HUVECs, as well as promoting their apoptosis.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yi F, Hao Y, Chong X and Zhong W: Overexpression of microRNA‑506‑3p aggravates the injury of vascular endothelial cells in patients with hypertension by downregulating Beclin1 expression. Exp Ther Med 15: 2844-2850, 2018
APA
Yi, F., Hao, Y., Chong, X., & Zhong, W. (2018). Overexpression of microRNA‑506‑3p aggravates the injury of vascular endothelial cells in patients with hypertension by downregulating Beclin1 expression. Experimental and Therapeutic Medicine, 15, 2844-2850. https://doi.org/10.3892/etm.2018.5733
MLA
Yi, F., Hao, Y., Chong, X., Zhong, W."Overexpression of microRNA‑506‑3p aggravates the injury of vascular endothelial cells in patients with hypertension by downregulating Beclin1 expression". Experimental and Therapeutic Medicine 15.3 (2018): 2844-2850.
Chicago
Yi, F., Hao, Y., Chong, X., Zhong, W."Overexpression of microRNA‑506‑3p aggravates the injury of vascular endothelial cells in patients with hypertension by downregulating Beclin1 expression". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2844-2850. https://doi.org/10.3892/etm.2018.5733