Open Access

Silencing of TGIF sensitizes MDA‑MB‑231 human breast cancer cells to cisplatin‑induced apoptosis

  • Authors:
    • Teng Pan
    • Tingting Mao
    • Haiyan Yang
    • Haiyu Wang
    • Yadong Wang
  • View Affiliations

  • Published online on: January 19, 2018     https://doi.org/10.3892/etm.2018.5780
  • Pages: 2978-2984
  • Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study was designed to explore the sensitivity of MDA-MB-231 cells to cisplatin after silencing the expression of TG‑interacting factor (TGIF) protein. Cell viability was measured using an MTT assay. Cell apoptosis was detected by the annexin V and dead cell assay and the Hoechst staining assay. Protein expression was analyzed using western blot analysis. A colony formation assay was also performed. It was observed that cisplatin reduced the expression of TGIF protein in a dose‑ and time‑dependent manner. Silencing TGIF significantly suppressed the cell proliferation and colony formation in MDA‑MB‑231 cells with the treatment of cisplatin. Results indicated that silencing TGIF could dramatically increase the cisplatin‑induced apoptosis rate in MDA‑MB‑231 cells. The expression of PARP and caspase‑3 proteins was correlated with the effect that silencing TGIF enhanced cisplatin sensitivity in MDA‑MB‑231 cells. The present data showed that silencing TGIF promoted apoptotic sensitivity that was induced by cisplatin in MDA‑MB‑231 human breast cancer cells and suggested that TGIF might be a therapeutic target for improving the chemotherapy response in triple‑negative breast cancer.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Pan T, Mao T, Yang H, Wang H and Wang Y: Silencing of TGIF sensitizes MDA‑MB‑231 human breast cancer cells to cisplatin‑induced apoptosis. Exp Ther Med 15: 2978-2984, 2018
APA
Pan, T., Mao, T., Yang, H., Wang, H., & Wang, Y. (2018). Silencing of TGIF sensitizes MDA‑MB‑231 human breast cancer cells to cisplatin‑induced apoptosis. Experimental and Therapeutic Medicine, 15, 2978-2984. https://doi.org/10.3892/etm.2018.5780
MLA
Pan, T., Mao, T., Yang, H., Wang, H., Wang, Y."Silencing of TGIF sensitizes MDA‑MB‑231 human breast cancer cells to cisplatin‑induced apoptosis". Experimental and Therapeutic Medicine 15.3 (2018): 2978-2984.
Chicago
Pan, T., Mao, T., Yang, H., Wang, H., Wang, Y."Silencing of TGIF sensitizes MDA‑MB‑231 human breast cancer cells to cisplatin‑induced apoptosis". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2978-2984. https://doi.org/10.3892/etm.2018.5780