Protective function of Bu Shen Huo Xue formula on the immunity of B6AF1 mice with experimental autoimmune premature ovarian failure

Wang,Peijuan; Lu,Yan; Chen,Si; Chen,Yue; Hu,Chunping; Zuo,Yawei

doi:10.3892/etm.2018.5804

Protective function of Bu Shen Huo Xue formula on the immunity of B6AF1 mice with experimental autoimmune premature ovarian failure

Authors:
- Peijuan Wang
- Yan Lu
- Si Chen
- Yue Chen
- Chunping Hu
- Yawei Zuo
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Affiliations: Department of Obstetrics and Gynecology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210028, P.R. China, Infirmary, 8724 Forces Health Teams, Wuxi, Jiangsu 214000, P.R. China
Published online on: January 29, 2018 https://doi.org/10.3892/etm.2018.5804
Pages: 3302-3310
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Autoimmune abnormality is one of the main causes of premature ovarian failure (POF). Bu Shen Huo Xue formula (BSHXF), a traditional Chinese medicine formula, has been clinically used for the treatment of patients with POF in China. Regulatory T cells (Tregs) are important in the pathogenesis of autoimmune POF. The aim of the present study was to evaluate the immunoprotective effects of BSHXF on POF and the underlying mechanisms. An experimental autoimmune POF model was induced in B6AF1 mice with zona pellucida 3 (ZP3) fragments. Following modeling, BSHXF (31.53 g/kg/day) was orally administered for 4 weeks. CD4+ T lymphocytes, Tregs, anti‑zona pellucida (anti‑ZP) antibodies and cytokines were detected using flow cytometry, enzyme‑linked immunosorbent assays, reverse transcription‑quantitative polymerase chain reaction and immunohistochemistry. The results revealed that BSHXF exhibited an immunoprotective function and reduced inflammatory cell infiltration and damage to the ovary. BSHXF upregulated the percentage of CD4+ CD25+ forkhead box P3+ T cells in the spleen, effectively inhibiting the activation of CD4+ T lymphocytes. The proliferation of Tregs was increased in serum obtained from mice in the BSHXF group in vitro. Anti‑ZP antibodies and interleukin‑10 and interferon-γ levels were decreased in the serum in the BSHXF‑treated group. The present study demonstrated that BSHXF had an immunoprotective effect on POF model mice. Additionally, it indicated that the protective mechanisms of BSHXF may be associated with an increase in Treg cells.

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