The association between different molecular weights of hyaluronic acid and CHAD, HIF-1α, COL2A1 expression in chondrocyte cultures

Sirin,Duygu  Yasar; Kaplan,Necati; Yilmaz,Ibrahim; Karaarslan,Numan; Ozbek,Hanefi; Akyuva,Yener; Kaya,Yasin  Emre; Oznam,Kadir; Akkaya,Nuray; Guler,Olcay; Akkaya,Semih; Mahirogullari,Mahir

doi:10.3892/etm.2018.5943

The association between different molecular weights of hyaluronic acid and CHAD, HIF-1α, COL2A1 expression in chondrocyte cultures

Authors:
- Duygu Yasar Sirin
- Necati Kaplan
- Ibrahim Yilmaz
- Numan Karaarslan
- Hanefi Ozbek
- Yener Akyuva
- Yasin Emre Kaya
- Kadir Oznam
- Nuray Akkaya
- Olcay Guler
- Semih Akkaya
- Mahir Mahirogullari
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Affiliations: Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Namik Kemal University, Tekirdag 59030, Turkey, Department of Neurosurgery, Istanbul Rumeli University, Tekirdag 59680, Turkey, Department of Medical Pharmacology, Istanbul Medipol University School of Medicine, Istanbul 34810, Turkey, Department of Neurosurgery, Namik Kemal University School of Medicine, Tekirdag 59060, Turkey, Department of Neurosurgery, Gaziosmanpasa Taksim Training and Research Hospital, Istanbul 34433, Turkey, Department of Orthopedics and Traumatology, Abant Izzet Baysal University School of Medicine, Bolu 14000, Turkey, Department of Orthopedics and Traumatology, Istanbul Medipol University School of Medicine, Istanbul 34214, Turkey, Department of Physical Medicine and Rehabilitation, Pamukkale University School of Medicine, Denizli 20070, Turkey, Department of Orthopedics and Traumatology, Medical Park Health Group, Istanbul 34180, Turkey, Department of Orthopedics and Traumatology, Denizli Private Hospital, Denizli 20070, Turkey, Department of Orthopedics and Traumatology, Memorial Health Group, Istanbul 34758, Turkey
Published online on: March 9, 2018 https://doi.org/10.3892/etm.2018.5943
Pages: 4205-4212
Copyright: © Sirin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the effects of three different formulations of hyaluronic acid (HA): Low molecular weight (MW) Sinovial One®, medium MW Viscoplus® and high MW Durolane®, on chondrocyte proliferation and collagen type II (COL2A1), hypoxia‑inducible factor 1α (HIF‑1α) and chondroadherin (CHAD) expression in primary chondrocyte cultures. Standard primary chondrocyte cultures were established from osteochondral tissues surgically obtained from 6 patients with gonarthrosis. Cell morphology was evaluated using an inverted light microscope; cell proliferation was determined with a MTT assay and confirmed with acridine orange/propidium iodide staining. Levels of CHAD, COL2A1 and HIF‑1α expression were assessed using specific TaqMan gene expression assays. The results demonstrated the positive effect of HA treatment on cell proliferation, which was independent from the MW. COL2A1 expression increased in the medium and high MW HA treated groups. It was observed that HIF‑1α expression increased in the high MW treated group alone. CHAD expression increased only in the medium MW HA treated group. Evaluation of gene expression revealed that levels of expression increased as the duration of HA application increased, in the medium and high MW HA treated groups. In terms of increased viability and proliferation, a longer duration of HA application was more effective. Taken together, it may be concluded that the administration of medium and high MW HA may be a successful way of treating diseases affecting chondrocytes in a clinical setting.

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