Effect of miR-146a-5p on proliferation and metastasis of triple-negative breast cancer via regulation of SOX5

Si,Chengshuai; Yu,Qiao; Yao,Yufeng

doi:10.3892/etm.2018.5945

Effect of miR-146a-5p on proliferation and metastasis of triple-negative breast cancer via regulation of SOX5

Authors:
- Chengshuai Si
- Qiao Yu
- Yufeng Yao
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Affiliations: Department of General Surgery, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research and Nanjing Medical University Affiliated Cancer Hospital, Nanjing, Jiangsu 210009, P.R. China
Published online on: March 9, 2018 https://doi.org/10.3892/etm.2018.5945
Pages: 4515-4521

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Abstract

MicroRNA (miR)-146a-5p functions as a tumor suppressor in various types of cancer. However, the role of miR‑146a‑5p in the development of triple‑negative breast cancer (TNBC) is unclear. The present study aimed to investigate the role of miR‑146a‑5p in TNBC. The expression level of miR‑146a‑5p in TNBC tissues and cell lines was initially detected using reverse transcription‑quantitative polymerase chain reaction. To predict the target gene of miR‑146a‑5p, TargetScan software was used and a dual luciferase assay was performed to verify the prediction. Furthermore, in order to explore the role of miR‑146a‑5p in TNBC, miR‑146a‑5p was overexpressed in TNBC cells using miR‑146a‑5p mimics. An MTT assay was performed to detect cell proliferation, and a Transwell assay was conducted to determine cell migration and invasion. Furthermore, western blotting was performed to measure associated protein expression. It was revealed that miR‑146a‑5p was downregulated in TNBC tissues and cell lines. SOX5 was indicated to be a target gene of miR‑146a‑5p and was upregulated in TNBC cells. Additionally, miR‑146a‑5p could inhibit TNBC cell proliferation, migration and invasion, repress the expression of mesenchymal markers (N‑cadherin, vimentin and fibronectin) and increase epithelial marker (E‑cadherin) expression. Furthermore, SOX5 overexpression eliminated the effects of miR‑146a‑5p mimics on TNBC cells. In conclusion, the data of the present study indicated that miR‑146a‑5p inhibits the proliferation and metastasis of TNBC cells by regulating SOX5.

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